Microscopy

Question 1

What is the formula for magnification?

Answer 1

Magnification= Power of eyepiece x Power of objective lens

Question 2

Formula for magnification?

Answer 2

Magnification: size of image/ size of object

Question 3

What is resolution?

Answer 3

The ability of a microscope to distinguish details in a sample or specimen.

Question 4

What do we use to measure a specimen in a microscope?

Answer 4

EYE PIECE GARTICULE

Question 5

How many sub divisions does the eyepiece graticule have

Answer 5

100

Question 6

Eye piece graticule?

Answer 6

This is a fine scale which fits into the eyepiece lens its units are arbitrary

Question 7

The stage micrometer

Answer 7

A scale fit into the coverslip the units of this scale are known.

Question 8

What’s the steps of calibrating the eye piece graticule with the stage micrometers?

Answer 8

1.align the eye piece graticule with the stage micrometer, parallel to one another horizontally.
2.count how many divisions on the eye piece graticule correspond to a set of divisions on the stage micrometer
3. Calculate how much one division equals using the stage micrometer and the eyepiece graticule
4.Count how many eye piece graticule divisions your specimen covers.

Question 9

How do I prepare a temporary slide?

Answer 9

1. Cut an onion between ur fingers
2. Using forceps add the thin epidermis layer on the slide
3. Add a drop of water and the cover slip
4. Add a drop of dye on the other side and filter paper on the other (irrigation technique)