MICROSCOPY

Question 1

the part of the microscope
that magnifies the image produced by
the microscope’s objective so that it can
be seen by the human eye

Answer 1

ocular lens

Question 2

to hold the objective and
ocular lenses at the two ends

Answer 2

body tube

Question 3

Holds the objective lenses &
attaches them to the microscope head.

Answer 3

nosepiece

Question 4

Supports the tube and connects it
to the base

Answer 4

arm

Question 5

magnifies the image

Answer 5

objective lens

Question 6

hold the slides in place

Answer 6

stage

Question 7

gather wavefronts from the
microscope light source and
concentrate them into a cone of light
that illuminates the specimen with uniform intensity over the entire
viewfield.

Answer 7

condenser

Question 8

used to vary the
intensity and size of the cone of light
that is projected upward into the slide

Answer 8

iris diaphragm lever

Question 9

allows for quick
focusing by moving the objective lens or
stage up and down

Answer 9

coarse-focus knob

Question 10

sharpen the focus
quality of the image

Answer 10

fine focus knob

Question 11

One
knob controls forward/reverse
movement of the slide. The other knob
controls right/left movement of the slide

Answer 11

Mechanical stage control knob

Question 12

responsible for shining
light on the object on the slide

Answer 12

light source

Question 13

It serves as a support for microscopes

Answer 13

base

Question 14

turns the illuminator off
and on

Answer 14

on/off switch

Question 15

When
transporting a microscope from one
location to another, always carry it with
both hands. Hold the microscope’s arm
with one hand and support the base with
the other. This prevents it from tilting or
dropping, reducing the risk of damage.

Answer 15

Proper Microscope Carrying

Question 16

Use the coarse
focus knob to initially bring the sample
into view, but do this gently. Once the
image is roughly focused, switch to the
fine focus knob for precise adjustments.
Avoid excessive force on these knobs as this could potentially damage the
slide or the microscope’s internal
components.

Answer 16

Focusing Technique

Question 17

Many
microscopes have adjustable light
intensity controls. If you need to change
the brightness of the light source, do so
gradually. Sudden, drastic adjustments
can cause the light to flicker or even
damage the bulb.

Answer 17

changing the light intensity

Question 18

When
switching between different objective
lenses, always begin by rotating the
nosepiece to the lowest magnification
lens. This provides maximum clearance
between the lens and the slide. Then,
rotate it to the desired objective gently,
without forcing it.

Answer 18

Objective Lens Rotation

Question 19

When placing a slide
on the microscope’s stage, ensure it is
properly centered and secured using the
stage clips or other securing
mechanisms if available. This prevents
the slide from shifting or falling during
observation, which could lead to
damage or inaccuracies in your
observations.

Answer 19

securing slides

Question 20

When carrying the microscope, ______________ - one hand
under the base and one on the arm

Answer 20

hold it
securely with two hands

Question 21

Keep the microscope _________ at all times

Answer 21

upright

Question 22

Keep microscope lenses clean with ___________. Do not use
paper towels or your clothing.

Answer 22

lens cleaner and lens paper

Question 23

Use only the ___________ when using
the high-power objective lens.

Answer 23

fine-focus knob

Question 24

Remove slides from the microscope
before _______.

Answer 24

putting it away

Question 25

Secure the cord with a ______________.

Answer 25

rubber band or wrap the cord gently around the microscope

Question 26

Store the microscope with the ___________ in place.

Answer 26

low-power objective lens

Question 27

Lower the ____

Answer 27

stage

Question 28

Put the microscope away in its ________.

Answer 28

proper location

Question 29

Begin by putting the slide on the microscope's stage that you wish to study. If your microscope has a mechanical stage, use those to hold it in place. If not, use the stage clips.

Answer 29

choose the slide

Question 30

Rotate the nosepiece to choose the lowest magnification objective lens (often 4x or 10x). The lowest magnification is often assigned to this objective lens on labels.

Answer 30

lowest magnification objective

Question 31

to give appropriate lighting for your sample. If there is a light intensity control, you can use it for this.

Answer 31

the light source should be adjusted

Question 32

Using the coarse and fine focus knobs, bring the sample into clear focus while looking through the eyepiece. To get close to the focus, start with the coarse adjustment knob and then fine-tune using the fine adjustment knob.

Answer 32

focus the sample

Question 33

Rotate the nosepiece to change to a higher magnification objective lens (for example, 40x or 100x) if you want to view the slide at a higher magnification. High magnification objectives should be used with caution as they have a shorter working distance and need for more exact focusing.

Answer 33

Higher Magnification Objectives

Question 34

To get exact focusing after changing to a higher magnification objective, you may need to refocus the sample using the fine adjustment knob.

Answer 34

refocus

Question 35

If your microscope includes changeable eyepieces, you may further _________ that best suits your needs. This helps you customize the overall magnification in particular.

Answer 35

enlarge the picture by choosing the eyepiece

Question 36

________________________ regarding the sample at each magnification level, then record your findings.

Answer 36

Make any required observations or notes

Question 37

Simply rotate the nosepiece back to the 4x or 10x objective to get back to the lowest magnification.

Answer 37

return to lowest magnification

Question 38

keep in mind to handle the microscope and slides ___________ to prevent damage.

Answer 38

delicately

Question 39

To prevent accidently harming the sample or the objective lenses, always ____________________ to find the sample and then progressively raise the magnification

Answer 39

start with the lowest magnification objective