Microscopy Flashcards
Creator of Microscope
Antoni Van Leeuwenhoek
Microscopes either have
1 lens or 2 lens or more
1 lens
Simple microscope (like magnification lens)
2 or more
compound microscope
Ocular lens magnification
10x or 20x
Scanner lens
4x
Low Power objective (LPO)
10x-20x
High Power Objective HPO
45x or 40x
Oil Immersion Objective
100x
Compoynd microscope
uses total magnification
total magnification formula
eyepiece x objective lens
Stereomicroscope/Dissecting microscope
Light source at top and bottom
dissecting needles
can have a drawing tube
Electron Microscope
does that exactly need light source
-electrons
-organisms can die
-view it in a computer
Two types of electron microscope
SEM- Scanning Electric Microscope
TEM- Transmission Electric Microscope
SEM
Scanning electric microscope
-looks at surface
TEM
transmission electric microscope
shows which structure is more dense than the other, internal
allows you to form a 3d image by diff atoms
Atomic Fore Microscopy
uses laser, much colorful bcs of flourescence
Confocal Microscopy
Bright field color
White
Dark field color
Dark
electron you have to understand
Phase contrast
Ocular micrometer
placed and accurate and known
Stage micrometer
Unknown, depends on objective, needs calibration
Calibration factor
SM10um)/OM
Actual size
(Om)(F)
supports whole microscope
base
elevates stage up and down
course adjustment knob
makes the picture clear
Fine adjustment knob
arm
holds
where the specimen is placed
stage
makes the image brighter
condenser
revolves objectives
nosepiece
iris diagphragm
adjust the amount of light passing thrhbthe condenser
