Microscopy Flashcards

1
Q

Creator of Microscope

A

Antoni Van Leeuwenhoek

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2
Q

Microscopes either have

A

1 lens or 2 lens or more

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3
Q

1 lens

A

Simple microscope (like magnification lens)

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4
Q

2 or more

A

compound microscope

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5
Q

Ocular lens magnification

A

10x or 20x

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6
Q

Scanner lens

A

4x

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7
Q

Low Power objective (LPO)

A

10x-20x

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8
Q

High Power Objective HPO

A

45x or 40x

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9
Q

Oil Immersion Objective

A

100x

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10
Q

Compoynd microscope

A

uses total magnification

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11
Q

total magnification formula

A

eyepiece x objective lens

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12
Q

Stereomicroscope/Dissecting microscope

A

Light source at top and bottom
dissecting needles
can have a drawing tube

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13
Q

Electron Microscope

A

does that exactly need light source
-electrons
-organisms can die
-view it in a computer

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14
Q

Two types of electron microscope

A

SEM- Scanning Electric Microscope
TEM- Transmission Electric Microscope

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15
Q

SEM

A

Scanning electric microscope
-looks at surface

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16
Q

TEM

A

transmission electric microscope
shows which structure is more dense than the other, internal

17
Q

allows you to form a 3d image by diff atoms

A

Atomic Fore Microscopy

18
Q

uses laser, much colorful bcs of flourescence

A

Confocal Microscopy

19
Q

Bright field color

A

White

20
Q

Dark field color

A

Dark

21
Q

electron you have to understand

A

Phase contrast

22
Q

Ocular micrometer

A

placed and accurate and known

23
Q

Stage micrometer

A

Unknown, depends on objective, needs calibration

24
Q

Calibration factor

A

SM10um)/OM

25
Q

Actual size

A

(Om)(F)

26
Q

supports whole microscope

A

base

27
Q

elevates stage up and down

A

course adjustment knob

28
Q

makes the picture clear

A

Fine adjustment knob

29
Q

arm

A

holds

30
Q

where the specimen is placed

A

stage

31
Q

makes the image brighter

A

condenser

32
Q

revolves objectives

A

nosepiece

33
Q

iris diagphragm

A

adjust the amount of light passing thrhbthe condenser