Microscopy Flashcards
Microscopy
the technical field of using microscopes to view objects and areas of objects that cannot be seen with the naked eye.
magnification
the ability to view an object as larger.
Resolution
the amount of detail you can see. It is the ability to measure the separation of images that are close together or the minimum distance between two objects at which the objects can just be distinguished as separate
Field of View
area viewed when looking into a microscope. The field of view is largest on the lowest power objective. When you switch to a higher power, the field of view closes in towards the center.
Compound light microscope
High magnification ranging from 40- 1000x;
Good for viewing thin sections of specimens that are micrometers in length and width: cells, protozoan, some bacteria;
Multiple lenses are able to reveal a great amount of detail in samples;
The light source is underneath the specimen while using a compound microscope.
Stereo/Dissecting Microscope
Very versatile: designed for viewing whole objects (3-dimensional) such as rocks, insects and flowers, but can also be used for viewing prepared slides;
Helpful tool for nature studies;
Lower power than compound microscopes;
Magnification levels range from 10 to 40x
Pocket/field microscope
High magnification;
Led light;
Compact and portable;
Aspheric lens system
SEM Scanning electron microscope
Can magnify over 2,000,000 x;
Electrons bounce off the specimen;
Produces a three dimensional image with great surface detail
TEM Transmitting Electron Microscope
a technique used to observe the features of very small specimens. The technology uses an accelerated beam of electrons, which passes through a very thin specimen that allows scientists to observe features such as structure and morphology.
Wet Mount
Temporary;
Made in the lab
Prepared slide
Permanent;
bought from a science supply store
