Microscopy Flashcards
1
Q
How do you set up a microscope?
A
Add sample to slide
Place cover slip and acetate grid over slide
Select lowest objective lens
Use course knob to focus
Use fine knob to bring image to sharp focus
Increase magnification refocusing at each level of magnification
2
Q
Scale grids
A
Used to estimate size of an object by comparing to grid size
3
Q
How to see plant nuclei
A
Iodine
4
Q
Animal nuclei
A
Methylene blue
5
Q
Bacteria cell wall
A
Crystal violet
6
Q
Total magnification equation
A
Eyepiece magnification x objective lens magnification
7
Q
Actual size equation
A
Image size / magnification
