Enzymes

Question 1

Describe the experiment to test for the conditions needed for amylase to digest starch

Answer 1

Add drop of iodine in a spotting tile
Add starch and amylase to a boiling tube
Remove a drop of the mixture every 10 seconds and add it to iodine on dropping tile.
At the point the amylase has fully digested the starch no colour change will be observed, note the time at which this occurs, repeat for other values of independent variable

Question 2

Adv of water bath

Answer 2

Constant temp

Reliable is temp is independent variable

Question 3

Rate of reaction equation

Answer 3

1/time

Question 4

Amylase

Answer 4

Used to break down starch to glucose

Question 5

Protease

Answer 5

Used to break frown proteins into amino acids

Question 6

Lipase

Answer 6

Used to break down fats into fatty acids and glycerol

Question 7

Effect of temperature on enzymes

Answer 7

As temp increases, reaction increases, until enzymes denature, then it decreases

Question 8

Effect of enzyme conc

Answer 8

Will increase until all substrate is used up the rate will fall to 0

Question 9

Effect of substrate conc

Answer 9

Will increase until all enzymes are used up

Question 10

Effect of pH

Answer 10

Different enzymes work best at different pH
If too high of or low bonds in amino acid of protein can break changing the active site, so substrate can no longer fit.
Optimum pH