Microscopy

Question 1

What are the three types of microscopes

Answer 1

Light(optical) microscope
Laser scanning microscope
Electron microscope

Question 2

Magnification

Answer 2

The degree to which an image is larger than the object itself. Image size over actual size.

Question 3

Reselution

Answer 3

The degree to which it is possible to distinguish between two objects are very close together

Question 4

Maximum effective magnification of light microscope

Answer 4

X1500 to x2000

Question 5

Minimum effective resolution of light microscope?

Answer 5

200nm

Question 6

Advantages of light microscopes

Answer 6

Cheap
Easy to use
Portable
Live specimen can be used

Question 7

Disadvantages of light microscopes

Answer 7

They use light which has a wavelength of 400-700nm therefore objects closer than 200nm apear as one (ribosomes)

Question 8

Magnification calculation

Answer 8

Image size over actual size

Question 9

Maximum effective magnification of EM

Answer 9

X1000000

X(1 million)

Question 10

Minimum effective resolution of EM

Answer 10

0.2nm