MICROSCOPIC EXAMINATION OF URINE Flashcards
Formed elements disintegrate rapidly in
Dilute, alkaline urine
May cause precipitation of amorphous crystals and other non pathologic crystals
Refrigeration
May dissolve some elements prior to centrifugation
Warming the specimen to 37 degrees celsius
Minimizes external contamination of the sediment
Midstream clean catch
Dilute, random specimen may cause
False negative readings
Specimen volume for microscopic examination
10 and 15 ml (average of 12 ml)
Centrifugation rcf and minutes
400 RCF for 5 minutes
Frequency of centrifuge calibration
Every three months
Frequency of centrifuge disinfection
Weekly
This should not be used to slow the centrifuge
Braking mechanism
All specimens must be capped to prevent
Biohazardous aerosols
Volume of the sediment after decantation
0.5 to 1.0 ml
Volume of sediment examined
20 microliter or 0.02 ml in 22 x 22 mm glass coverslip
Allowing the specimen to flow outside of the coverslip may result in the loss of heavier elements such as
Casts
To control sediment volume
Decanting pipettes