microscopic Flashcards
formed elements
cells
casts
crystals
mucus
blood indicates
RBCs and RBC casts
protein indicates
casts, cells
nitrite indicate
bacteria
glucose indicates
yeast
less urine=
less sediment
make sure to spin down specimen
examination tech
brightfield- standard
polarizing- birefringent
Hematuria
dysmorphic rbc associated= glomerular damage
normal rbc= recent damage
most important WBC to ID
granulocytes
eosionophils require staining in
acute DI interstitial nephritis
correlation for WBC
+ leukocyte
+nitrite
hazy turbid
large numbers of epis indicate
irritation or infection
correlation for squamous epi
hazy turbid urine
report average for 5 LPF
what can transitional cells indicate
infection, irritation higher in tract, or catheter placement
correlates with transitional epi
hazy turbid urine
elevated squamous
blood +
highly significant cell found
renal tubular epi
suggest glomerular disease, acute tubular necrosis
renal tubular epi
higher N:C ration
-help reabsorb substances
RTE with large amounts of fat called
oval fat bodies
if a lot of cholesterol= maltese cross under polarized
oval fat bodies associated with
nephrotic syndrome, lipid storage, diabetes
stain in sudan oil
RTE correlates
hazy turbid
protein+
blood +
bili+
lipid droplets
bacteria presence indicates
UTI
bacteria correlates
leukocyte +
nitrite +
alkaline pH
mucous
where is yeast commonly found
diabetic patient because yeast rapidly ferment glucose
most common parasite found in urine
trichomonas
needs to be unrefrigerated
need to ID motile
when is sperm found
men who recently ejaculated
when is sperm clinically significant
retrograde emission - semen ejected in bladder during ejack.
or infertility
correlates sperm
protein +
make sure if sperm found in minor
to report
what is mucous
threads of protein - uromodulin
no significance
protein +
what is a cast
precipitated protein that holds shape of convoluted tubules
hyaline casts made of
uromodulin
small amount may be present after exercise or stress
large amounts- glomerulonephritis, blah
how do hyaline cast get missed
high light, low contrast
how to report hyaline casts
average number for low power field
-insept at high
granular casts formed
lysozyme excreted by RTE’s into hyaline casts or degrading cellular casts
what is granular cast associated with
tubular interstital disease and graft rejection
all casts how to report
count average in 5 low power, but confirm on high
waxy casts
granular casts degrade into waxy
-jagged edges or tears
associated with renal failure
highly significant
rbc cast
rbc trapped in cast from glomerulus
associated with glomerulonephritis
significant!!
if find a rbc cast with lipid or protein think
nephrotic syndrome
wbc casts
wbc trapped in cast from nephron and suggest inflammation of upper urinary tract
bacterial cast
bacteria trapped in matrix
-pyelonephritis
correlates with + leukocyte esterase
epithelial cast
RTE cells present in cast matrix
-same disorders as RTE cells
highly significant
fatty casts
lipid droplets or oval fat bodies trapped in cast
associated with nephrotic syndrome, toxic tubular necrosis, diabetes, crush injuries
highly significant
what does fatty cast not stain with
sternheimer-malbin stains
mixed cellular cast
more than one cellular element trapped in cast
crystal formation is dependent on
temp and pH
best urine to observe crystals
fresh unpreserved
uric acid
normal crystal
-acidic
-rhomboid shape
can be increased in gout - correlate with serum uric acid
amorphous uric acid
brownish or pink
calcium oxalate
-normal crystal
-acidic
renal calculi
-tomatoes
kidney stones are this
calcium oxalate form
dihydrate
shape is envelope
what form of calcium oxalate associated with antifreeze poisoning
monohydrate
dumbell
triple phosphate
-normal crystal
-alkaline
coffin lid
calcium phosphate crystal
alkaline urine
-so many shapes; rosettes
what can calcium phosphate crystals dissolve in
weak acetic acid
calcium carbonate crystal
alkaline urine
oblong dumbell shape
ammonium biurate
thorny apple
-old urines : means urine is unacceptable
normal crystal
cystine crystal
abnormal
seen in cystinuria: cystine can’t be reabsorbed by renal tubule
kidney stones from young age
how to confirm cystine crystals
cyanide- nitroprusside test
shape of cystine crystals
thick, hexagonal crystals
tyrosine crystals
abnormal
liver disorders and inborn aminoacidopathies
found with leucine
shape of tyrosine crystal
fine needles
leucine crystal
abnormla
liver disorders
tree stump
bilirubin crystals
abnormal
liver disorders
dark amber rosettes
cholesterol crystal
seen with lipiduria
oval fat bodies
notched corners
starch granules have
pseduo-maltese cross