Microscopes Flashcards

1
Q

What does the objective lens do?

A

Magnified s the specimen on the slide

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2
Q

What does the coarse focussing lens do?

A

Adjusts distance between lens and stage

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3
Q

What does the fine focussing wheel do?

A

Makes slight adjustments

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4
Q

Magnification

A

How many times larger an image obtained using a microscope is, relative to the real object

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5
Q

Resolution

A

The smallest distance between two points that can still be seen as two points

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6
Q

100cm has …. millimetres

A

1000mm

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7
Q

1mm has …. micrometers

A

1000 micrometers

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8
Q

1cm has …. micrometers

A

10 000 micrometers

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9
Q

1000mm has …. micrometers

A

1 000 000 micrometers

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10
Q

Magnification equation

A

Size of image/actual size

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11
Q

What are the advantages of an electron over a light microscope?

A
  • Higher magnification
  • Higher Resolution
  • Used to examine surface structures of cells/structures
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12
Q

What can a light microscope do that a electron microscope cannot?

A

Examine living cells.

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13
Q

What is the formula to calculate total magnification?

A

Total = eyepiece lens magnification x objective lens magnification

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14
Q

Millimeter As a Meter in Standard Form

A

x10^-3 m

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15
Q

Micrometer as a meter in standard form

A

X 10^-6 m

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16
Q

Nanometer As a Meter in Standard Form

A

X10^-9 m

17
Q

Picometer as a Meter in Standard Form

A

x10^-12 m

18
Q

How does a light microscope work?

A

Passes light through a specimen, to see it in a greater magnification than the human eye

19
Q

How does an lector’s microscope work?

A

Electrons are passed through just the specimen

20
Q

What type of structures can be seen through an electron microscope?

A

Sub-cellular structures

eg: mitochondria, chloroplasts, ribosomes and their functions