Microscopes Flashcards
Are atoms microscopic? Why?
No, because you can’t see them with a microscope, they’re too small
What does it mean if something is biological?
It comes from a living thing
Where is protein synthesis found?
In ribosomes
What is the equation to work out the actual size of an image viewed through the microscope?
Actual size = image size / magnification
What does image size mean?
Size of the picture (measure it)
What is magnification?
How much bigger the image is than the actual size
What is the equation to work out image size?
Image size = actual size x magnification
What is the equation to work out magnification?
Magnification + image size / actual size
What is resolution?
The smallest distance between two points that can be seen as two points
Why do we stain specimen cells with iodine?
So we can see them more easily.
What do we put before the magnification when writing magnification?
An x sign
In a cells, where are the ribosomes usually found?
Near the nucleus
Who created the light microscope?
Robert Hooke
What was the magnification of Robert Hookes microscope?
X30
If the magnification is x30, how much bigger will the image viewed be than actual size?
30 times
What are the two types of lens in a modern light microscope?
Eyepiece lens and objective lens
If an eyepiece lens has a magnification of x10 and the objective lens has a magnification of x4 what is the total magnification?
X40
What is the field of view?
What you see through the microscope
What do electron microscopes allow us to see?
Sub cellular structures
What are micrographs?
Computer generated images from an electron microscope
Four steps to using a microscope
1) Place the slide on the stage and look through the eyepiece lens
2) Make sure the smallest objective lens is on
3) Turn the focusing wheel to get a clear image
4) Increase the magnification of the objective lens
On a microscope, how can we identify the objective lens?
The small red one
On a microscope, how can we identify the focus wheel?
The big one
How do we carry a microscope?
Keep one hand on base and one hand on grip
Three simple steps to getting a specimen on a slide ready for viewing?
1) Place a drop of iodine solution on the slide
2) Place specimen on drop on the slide
3) Gently lower a coverslip onto the slide at a 45* angle
What are three important rules to use when drawing a biological drawing?
Continuous lines - no sketching
Label lines don’t cross, no arrow heads, drawn with ruler
No shading or colouring
