microscopes Flashcards
what can you see with light microscope
can see large cellular structures
X1,500
types of electron microscope
transmission - electrons through specimen
INTERNAL features
X500,000
scanning - electrons bounced off surface providing 3D
EXTERNAL features
X100,000
newest microscope
laser scanning confocal microscope
cells stained with fluorescent dyes
scanned with laser beam which is reflected by dyes
- focus at specific depth so images in more depth and not blurry
- images at different depths and using computer form 3d image
magnification is…
enlargement of an image
resolution is…
ability to differentiate between two separate points
increases clarity
magnification =
eyepiece lens x objective lens
staining for light microscopes
coloured dyes stain tissue
makes structures visible
differential staining
staining for transmission microscopes
stains that absorb particles
use heavy metal compunds
image size =
actual x magnification
