microscopes

Question 1

what can you see with light microscope

Answer 1

can see large cellular structures
X1,500

Question 2

types of electron microscope

Answer 2

transmission - electrons through specimen
INTERNAL features
X500,000

scanning - electrons bounced off surface providing 3D
EXTERNAL features
X100,000

Question 3

newest microscope

Answer 3

laser scanning confocal microscope
cells stained with fluorescent dyes
scanned with laser beam which is reflected by dyes
- focus at specific depth so images in more depth and not blurry
- images at different depths and using computer form 3d image

Question 4

magnification is…

Answer 4

enlargement of an image

Question 5

resolution is…

Answer 5

ability to differentiate between two separate points
increases clarity

Question 6

magnification =

Answer 6

eyepiece lens x objective lens

Question 7

staining for light microscopes

Answer 7

coloured dyes stain tissue
makes structures visible

differential staining

Question 8

staining for transmission microscopes

Answer 8

stains that absorb particles
use heavy metal compunds

Question 9

image size =

Answer 9

actual x magnification