Microscopes Flashcards

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1
Q

This is easy you stupid shit: What can you see with an electron microscope that you cannot see with a traditional light microscope?

A

Bacteria, Viruses, molecules, atoms in crystals.

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1
Q

Alright dumb fuck: Why are photons not suitable for viewing objects smaller than atoms?

A

Photons are too large and have wavelengths that are not short enough to resolve objects smaller than atoms.

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2
Q

Alright stupid ass motherfucker: What replaces photons in an electron microscope to enable higher resolution imaging?

A

Electrons, which have a much shorter equivalent wavelength.

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3
Q

Alright Mr. get-no-bitches, How do Electron Microscopes Work
What are the four main components of an ordinary microscope, and what are their equivalents in an electron microscope?

A

Light source (replaced by electron beam), specimen (needs vacuum), lenses (replaced by electromagnets), and the image (electron micrograph or screen image).

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4
Q

Since you can’t pull at all, How do electron microscopes achieve magnification?

A

By bending electron beams with electromagnets instead of using lenses to bend light.

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5
Q

You stupid fuck you need an electron microscope for that shrimp size brain of urs: Types of Electron Microscopes
What distinguishes a Transmission Electron Microscope (TEM) from a Scanning Electron Microscope (SEM)?

A

TEMs image the insides of materials by transmitting electrons through them, while SEMs image surfaces by scanning them with electrons.

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6
Q

Bitch, listen: How does a Scanning Tunneling Microscope (STM) differ from TEMs and SEMs in terms of functionality and specimen damage?

A

STMs use a sharp metallic probe to scan surfaces at atomic levels without high-energy electron beams, reducing specimen damage.

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7
Q

Lazy ass mf
Define ‘micrograph’.

A

A photo taken through a microscope.

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8
Q

You stupid mf: What does magnification mean in microscopy, and how is it different from resolution?

A

Magnification is enlarging the image of an object, while resolution is the ability to distinguish between two closely situated objects.

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9
Q

Dumb little shit: How is contrast enhanced in microscopy?

A

By using stains or fluorescent substances to distinguish structures against the background.

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