Microscopes Flashcards

1
Q

What is magnification?

A

The factor by which an image has been enlarged.

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2
Q

What is resolution?

A

The ability to see closely placed objects as separate structures.

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3
Q

What are the key features of a light microscope?

A
  • specimens are illuminated with light.
  • specimens can be living or dead.
  • often need to be stained to be visible and to be thin.
  • Use several lenses to obtain a high magnification.
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4
Q

What are the key features of TEM’s?

A
  • Produces a beam of electrons which form an image on a screen.
  • uses a dead specimen.
    -Uses very thin specimens.
  • Produces black and white images
  • produces 2D images.
  • shows the inside of structures.
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5
Q

What are the key features of SEM’s?

A
  • Scans a fine beam of electrons onto a specimen and collects the electrons scattered
  • Has the best resolution
  • Shows the surface of cells
  • Dead samples
  • Produces coloured images
  • Produces 3D images
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