Microscopes Flashcards

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1
Q

Mag. Def.

A

Making image larger the the real thing

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2
Q

Stain

A

Increases contrast

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3
Q

Resolution

A

Clarity of image

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4
Q

Iodine

A

Plant/Methylene blue - animals

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5
Q

Triangle

A

I

A M

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6
Q

Microscope Total Mag. =

A

Eyepiece Lens x Objective Lens

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7
Q

MAG(Drawing)=

A

I/A ~ Image/Actual Size

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8
Q

A(Actual Size)=

A

I/M - Image size/Magnification

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9
Q

Dis. Light Mic.

A

Limited Mag&Res

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10
Q

Dis. Electron Mic

A

Can only study dead cells
Can only be seen in black and white
Expensive

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11
Q

Ads. Light Mic.

A

Portable
Easy to use
Can see living cells
No harsh chem. to be used

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12
Q

Ads. Electron Mic.

A

Greater Mag&Res

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13
Q

How does the light Mic. see it’s specimen?

A

Light Rays

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14
Q

How does the Electron Mic. see it’s specimen?

A

Beams of high speed electrons to produce an image on flourescent screens

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15
Q

How does the light Mic. focus on the specimen?

A

Glass Lense

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16
Q

How does the electron Mic. focus on it’s specimen?

A

Electromagnets

17
Q

Explain why ribosomes are not visible using a light microscope.

A

Ribosomes have a diameter of 25nm which is smaller than half. The wavelength of visible light and the light microscope only has a resolution of 200nm.