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BSC 2086 Lab > Microscope use > Flashcards

Microscope use Flashcards

1
Q

important factors affecting image quality of microscope

A

enhancing resolution

contrast

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2
Q

resolution def

A

ability to see details clearly

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3
Q

contrast def

A

distinction between light and dark

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4
Q

microscopes on campus are

A

monocular compound light microsopes

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5
Q

monocular def

A

single eyepiece

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6
Q

compound microscope def

A

image on a slide will pass through more than one lens

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7
Q

ocular lens def

A

in eyepiece

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8
Q

objective lenses

A

selected from one of the objectives

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9
Q

mechanical stages feature

A

allow slides to be moved easily with knobs

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10
Q

parafocal feature

A

the images stay nearly in focus when objective lensees are switched

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11
Q

paracentric feature

A

once a sample is centered in the field of view, it will remain centered when switching to a higher magnification

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12
Q

brightness control knob def & function

A

on/off switch at base of microscope
turning it will increase illumination from light bulb
recommend- turn it to 3/4 to end point to avoid creating a field that is too bright to regulate

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13
Q

stage def

A

slide will be placed on this platform for viewing

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14
Q

slide holder and claw

A

claw needs to be opened to position slide

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15
Q

“X” & “Y” travel knobs

A

moves slide horizontally and vertically

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16
Q

ocular lens and magnification

A

magnifying lens in eyepiece

magnifies image by 10X

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17
Q

revolving nosepiece

A

disc is rotated when selecting and objective lens

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18
Q

objective lenses

A

magnifying lenses used to further magnify the image

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19
Q

magnification of each objective lense is

A

multiplied by 10, power of ocular lenses, to determine total magnification

20
Q

red lense magnification

A

Lens Power Ocular Power Magnification

4X 10X 40X

21
Q

yellow lense magnification

A

Lens Power Ocular Power Magnification

10X 10X 100X

22
Q

red lense name

A

scanning

23
Q

yellow lens name

A

low-power

24
Q

blue lense magnification

A

Lens power ocular power magnification

40X 10X 400X

25
Q

blue lens name

A

high-power

26
Q

white lens magnification

A

lens power ocular power magnification

100X 10X 1000X

27
Q

white lens name

A

oil immersion

28
Q

order of magnification of lenses

A

red- scanning
yellow- low- power
blue- high-power
white- oil immersion

29
Q

coarse and fine focus knobs function

A

used to adjust height of stage to bring the slide closer to the objective lens

30
Q

coarse focus knob used with which lens

A

lower power lenses

31
Q

coarse knob does

A

moves stage in larger increments than fine adjustment knob

32
Q

coarse knob should not be used when

A

the two higher power lenses are in place

33
Q

coarse and fine focus knob should never

A

be used at the same time

34
Q

no two adjustment knobs should

A

be used simultaneously

35
Q

iris diaphram def and function

A

lever located approx 1.5 in under stage

used to regulate the amount of light reaching the slide

36
Q

substage condenser def and function

A

does not magnify

condenses image so it can be seen

37
Q

substage condenser should not

A

be adjusted for routine use of microscope

38
Q

as power of lens inscreases, resolution…

A

decreases

39
Q

why does resolution decrease when power of lens increases

A

smaller surface area of stronger lenses

refraction of light waves as they pass between glass surfaces

40
Q

use of oil immersion in between slides does what

A

less light refracted

image quality improves

41
Q

immersion oil has almost the same refractive index as

A

glass, light behaves similarly when passing through both mediums

42
Q

use of oil allows

A

to magnify specimen 1000X with some clarity

43
Q

using 1000X lens without oil

A

very unclear image due to excessive light refraction

44
Q

steps of use of highest power objective lens, oil immersion

A
  1. use nosepiece to postition scanning lens on one side of slide and oil immersion lens on other side
  2. apply drop of immersion oil to slide, using incoming ligth as target
  3. rotate nosepiece to click immersion lens in place over slide
  4. use fine focus knob and iris diaphragm to improve image
  5. can go back and forth between scannning power and oil lens to refine image
  6. immersion oil lens makes contact with drop of oil
45
Q

if you lose focus with oil immersion go back to

A

scanning power, back and forth

BSC 2086 Lab (5 decks)

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