Microscope & Lab Safety QUIZ

Question 1

The microscope may function poorly with the accumulation of
a. Dust
b. Immersion oil
c. Biologic contamination
d. All choices

Answer 1

All choices

Question 2

Which of the following is NOT a type of light microscope?
a. Dark field
b. Bright-field
c. Phase contrast
d. Fluorescence

Answer 2

Fluorescence Microscope

Question 3

Regarding the principle of diffraction, when light passes through small apertures it will cause
a. Large, diffuse images
b. Magnification no <400x
c. Image quality to be unchanged
d. Large, sharp images

Answer 3

Large, diffuse images

Question 4

What is/are the potential hazard(s) of working with biological materials in a veterinary laboratory?
a. Prions
b. Toxins
c. Parasites
c. All choices

Answer 4

All choices

Question 5

Just a reminder to know the parts of the microscope

Answer 5

You’re doing great!

Question 6

T/F: Electron microscopes utilize the interaction of photons with the specimen to generate an image.

Answer 6

False. It uses the interaction of ELECTRONS

Question 7

T/F: The purpose of immersion oil is to improve the detail of the image at very high magnification.

Answer 7

True

Question 8

T/F: One advantage of the Phase Contrast Microscope over the Bright Field, is the ability to visualize the detailed structures of live micro-organisms.

Answer 8

True

Question 9

The condenser should be kept in its ____________position for hematology evaluation (needs the most light).
a. Lowest
b. Highest

Answer 9

Highest

Question 10

________________use the coarse adjustment knob for high magnification objectives.
a. Never
b. Always

Answer 10

Never

Question 11

______light tends to improve contrast.
a. Dim
b. Bright

Answer 11

Dim

Question 12

To increase brightness, open the ___________________lever.
a. Nosepiece
b. Diaphragm

Answer 12

Diaphragm

Question 13

When the 10x objective is used on a microscope, the total magnification of the specimen is _______.
a. 100x
b. 1x
c. 1000x
d. 10x

Answer 13

100x

Question 14

Describe proper PPE for working with specimens of biological hazard potential.

Answer 14

Goggles, gloves, a gown that can be disposed of, mask & hair net if necessary

Question 15

Describe the BTC Vet Tech Lab Safety Protocol for working with specimens of biologic hazard potential. (Do not list the PPE of the previous question)

Answer 15

1. Hair back
2. Belongings under desk
3. No jewelry or applying makeup
4. No eating/drinking
5. Sanitizing surfaces before/after using & covering properly when handling biohazards
6. Thoroughly washing hands before/after lab