Microscope

Question 1

Compound bright - field microscope

Answer 1

• common microscope used in the clinic
• has 2 lens system

Question 2

What are the two-lens system?

Answer 2

• the ocular ( eyepiece) which is the nearest to the eyes.
• the objective which is the nearest object being viewed.

Question 3

Oculars

Answer 3

• binocular ( the two eyepieces )
• located at the top if the microscope
• already magnified at 10x
• but can also be available in 15x and 20x

Question 4

Nose piece

Answer 4

• underside of microscope that revolves with different objective that are attached
• has 3 objectives

Question 5

What are the three objectives

Answer 5

• low power ( 10x )
• high power ( 40x OR 43x 45x )
• Oil immersion lens ( 95x, 97x OR 100x but the 100x is common)

Question 6

Low power

Answer 6

• 10x but when viewed with the ocular it is 100x ( multiply 10 x with 10 x )
• used for initial viewing object / locate object
• use course knob to bring object into focus
• use low light

Question 7

High power

Answer 7

• 40x ( 400 x)
• used for greater magnification
• fine adjustment is used to bring object into sharp focus
• use more light

Question 8

Oil - immersion

Answer 8

• 100x ( 1000x)
• used to view stained objects on side
• highest magnification of all bright field objectives
• drop of immersion oil in order to view object.
• use maximum light

Question 9

What is the degree of magnification

Answer 9

• this is where we multiple the ocular with the objective
• Ex. 10x x 10x = 100x
• 10x x 40x = 400x

Question 10

What is a resolving power ?

Answer 10

• ability of a microscope to distinguish between two separate but closely spaced details object being viewed.
• determined by the quality of the objective lens

Question 11

Light source

Answer 11

• located in the base of the microscope
• supplies light to the objective viewed

Question 12

Condenser

Answer 12

• condenser is located under the stage
• it focuses or directs the available light into the objective as it is raised or lowered

Question 13

Iris diaphragm

Answer 13

• located in the condenser
• allows how much light is entered when object being viewed.

Question 14

Coarse adjustment knob

Answer 14

• used to focus w/ low - power objective ONLY

Question 15

Fine adjustment knob

Answer 15

• used to give a sharper image after the object is brought into view with the coarse adjustment

Question 16

Working distance

Answer 16

• distance between the objective and the slide when the object is in sharp focus

Question 17

Stage

Answer 17

• stage is the support for the object being viewed.

Question 18

Parfocal

Answer 18

• when switching from low objective to high power you’d have to refocus object but only slightly using the FINE adjustment knob

Question 19

Proper lighting adjustment

Answer 19

• low power = low light / condenser lowered to reduce brightness / iris diaphragm closed
• high power = light / condenser raised / iris diaphragm opened
• oil immersion = high light / condenser raised all the way / iris diaphragm completely open to give max. light.

Question 20

How should a microscope be cleaned?

Answer 20

• lens paper to soak up oil
• then lens cleaner to clean off rest of residue

Question 21

How to transport ?

Answer 21

• held securely, one hand on the base and the other on the arm

Question 22

How should it be stored?

Answer 22

• placed in low power objective
• nosepiece in lowest position
• stage centered
• cord wrapped and then out away.