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Microbiology Lab > Microscope > Flashcards

Microscope Flashcards

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Biology 101 flashcards
1
Q

enables us to observe and identify the minute organisms

A

microscopy

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2
Q

use of microscopy in microbio

A
  • identify microorganisms by observing their features
  • different type of microscope serve different functions to characterize microorganisms
  • understanding the biology of microorganisms
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3
Q

Types of microscope

A
  1. Compound
  2. Darkfield
  3. Electron
  4. Fluorescence
  5. Phase Contrast
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4
Q

has two lenses (objective and ocular) with source of light that can either be natural and artificial

A

compound microscope

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5
Q

LPO

A

10x

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6
Q

HPO

A

40x

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7
Q

Oil Immersion Objective

A

100x

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8
Q

Scanning Objective

A

4x

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9
Q

What are the sources of light of compound microscopes?

A

visible light (artificial or natural light)

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10
Q

has a special field type of condenser that scatters the light

A

darkfield microscope

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11
Q

illuminate unstained specimen against a dark background

A

darkfield microscope

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12
Q

ues beam of electrons (light) to illuminate specimens

A

electron microscope

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13
Q

provides a high resolution of biological and non-biological samples

A

electron microscope

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14
Q

uses ultraviolet light to illuminate speciments

A

fluorescence microscope

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15
Q

detection of specific structures within a cell with the use of a specific fluorescent dye

A

fluorescent microscope

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16
Q

what type of objective is best when viewing under fluorescence microscope

A

OIO

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17
Q

best to use in observing microorganisms in their natural state

A

phase contrast microscope

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18
Q

has a special condenser that enables the observation inside the cell

A

phase contrast microscope

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19
Q
A
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20
Q

what is the best slide to use in phase contrast microscope?

A

hanging drop slide

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21
Q

stain or unstain

compound microscope

A

stain/not stain

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22
Q

stain or unstain

darkfield microscope

A

not stained

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23
Q

stain or unstain

electron microscope

A

stain/not stain

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24
Q

stain or unstain

fluorescence microscope

A

stained

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25
Q

stain or unstain

phase contrast microscope

A

unstained

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26
Q

type of parts of the microscope

eyepiece

A

mechanical

27
Q

type of parts of the microscope

diopter adjustment

A

mechanical

28
Q

type of parts of the microscope

revolving nosepiece

A

mechanical

29
Q

type of parts of the microscope

objective lenses

A

magnifying

30
Q

type of parts of the microscope

mechanical stage

A

mechanical

31
Q

type of parts of the microscope

stage clips

A

mechanical

32
Q

type of parts of the microscope

diaphragm

A

illuminating

33
Q

type of parts of the microscope

condenser

A

illuminating

34
Q

type of parts of the microscope

illuminator (light source)

A

illuminating

35
Q

type of parts of the microscope

head

A

mechanical

36
Q

type of parts of the microscope

arm

A

mechanical

37
Q

type of parts of the microscope

coarse adjustment knob

A

mechanical

38
Q

type of parts of the microscope

fine adjusment knob

A

mechanical

39
Q

type of parts of the microscope

light switch

A

mechanical

40
Q

type of parts of the microscope

base

A

mechanical

41
Q

type of parts of the microscope

ocular lenses

A

magnifying

42
Q

When viewing under scanning and LPO, what adjustment knob should you use?

A

coarse adjustment knob

43
Q

when viewing under HPO and OIO, what adjustment knob should you use?

A

fine adjustment knob

44
Q

distance between objective and slide

A

working objective

45
Q

true or false

the lower magnification, the higher working distance

A

true

46
Q

true or false

the higher magnification, the higher working distance

A

false

magnification is inversely proportional to working distance

47
Q

number that expresses the ability of a lens to resolve fine detail in an object being observed

A

numerical aperture

48
Q

true or false

the higher the magnification, the higher numerical aperture

A

true

49
Q

true or false

the higher the magnification, the lower numerical aperture

A

false

magnification and NA are directly proportional with each

50
Q

What is the numerical aperture of:

4x (scanning)

A

0.1

51
Q

What is the numerical aperture of:

10x (LPO)

A

0.25

52
Q

What is the numerical aperture of:

40x (HPO)

A

0.65

53
Q

What is the numerical aperture of:

100x (OIO)

A

1.25

54
Q

color of objective lenses

scanning

A

red

55
Q

color of objective lenses

LPO

A

yellow

56
Q

color of objective lenses

HPO

A

blue

57
Q

color of objective lenses

OIO

A

white

58
Q

range of distance for which the specimens (objects) is imaged with an acceptable sharpness on the image plane

A

depth of focus

59
Q

true or false

depth of focus is inversele proportional to NA

A

true

60
Q

depth of specimen layer which is in sharp focus at the same time

A

depth of field

61
Q

true or false

depth of field is inversely proportional to NA

A

true

62
Q

ability of microscope to distinguish s specimen/sample

A

resolution

63
Q

true or false

resolution is inversely proportional to NA and directly proportional to depth of field

A

true

Microbiology Lab (7 decks)

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