Microscope Flashcards
Use to magnify the specimen w/ magnification of 10x
Eyepiece/Ocular lenses
It connects the eyepiece and the head of a microscope. Holds the eyepiece in place.
Eyepiece tube
It is attached to the nosepiece of the microscope, and w/ the magnification varying from 4x, 10x, 40x, 100x.
Objective lenses
A structural part where it is use to carry the microscope. It connects the base to the nosepiece and the eyepiece of the microscopes.
Arm
It holds the objective lenses in place, also it helps the objective lenses to rotate for you to choose which magnification level you want to use.
Nosepiece
It holds the slide to prevent it from moving.
Clip holder/ Stage clips
It is where the specimen is placed for observation. And also it has a hole in the middle, where the light to pass through the slide.
Stage
Used to view the initial focus of the specimen, used w/ low power objective.
Coarse adjustment screw
Used to view the final and clear focus of the specimen, used w/ high power objective.
Fine adjustment screw
A whole in the stage where light can pass through.
Aperture
The light source of the microscope. It reflect light from an external source up through the bottom of the stage.
Mirror/Illuminator
Supports the microscope and it’s where illuminator is located.
Base
Gathers and focuses light from the illuminator onto the specimen being viewed.
Condenser
Adjusts the amount of light that reaches the specimen.
Diaphragm/ Iris
These knobs move the stage left and right or up and down.
Stage control
Useful as a means to change focus on one eyepiece so as to correct for any difference in vision between your two eyes.
Diopter adjustment
The ‘‘father of microscopes’’ Dutchman, early 1670s
Anton van Leeuwenhoek
Who (Dutch) experimented w/ a crude concept of microscope with magnification level of 10x to 30x or so, 1590.
Zaccharias Janssen and his son Hans
Who (Italian) improved the principle of lenses and added a focusing device to improve somewhat upon what the Janssen’s had done.
Galileo
Who was the first person to see and describe the bacteria, yeast, plants, and life in a drop of water with the magnification of up to 270x, 1674.
Anton van Leeuwenhoek
High power microscopes
compound
Low power microscopes
Stereo or dissecting
High power microscopes can have a magnification level of?
40x to 1000x and some can go up to 1500x or 2000x.
It is the most used light method. Light is projected from below to pass through the specimen.
Trans illumination
Have a single light path that produce 2-D image of the specimen
Compound Microscopes
There a two separate light paths that produce 3-D image of the specimen.
Stereo microscopes
Uses the differences in the phases of light transmitted or reflected by a specimen to form distinct contrasting images of different parts of the specimen. Transparent and colourless images.
Phase Contrast
The object viewed is illuminated by polarized light for typically analyzing the content and make-up of organic and inorganic material.
Polarizing
Combination of a microscope and digital camera. Have high power level.
Digital
New technology for a miniature and illuminator in one unit. Low power level.
Handheld digital Microscopes
Uses a beam of accelerated electrons as a source of illumination. It has a high resolution of images of biological and non-biological specimens.
Electron Microscopes
Used to view thin specimens which electrons can pass through a projection image used to image the interior of a cell.
Transmission electron microscope (TEM)
Depends on the emission of secondary electrons from the surface of a specimen. Provide detailed images of the surface of the cell. Also used in particle counting, and size determination.
Scanning electron microscope (SEM)
The number of times the image is larger than the object would appear to the naked eye.
Magnification (power)
allows for brighter, sharper images combined with a wide field of view.
Low powers
Often for examining slide specimens, present larger but dimmer images with a narrower field of view.
Higher powers
When the magnification is increased but the detail is not (due to the limitation of the resolution), it is called?
Empty magnification
A condition in which all optical elements are centered on the same axis.
Collimation (Alignment)
