Microscope

Question 1

head

Answer 1

• upper part of frame
• supports lenses
• also called body tube

Question 2

arm

Answer 2

• vertical part of frame
• connects head and base

Question 3

light switch

Answer 3

• turns lights on and off

Question 4

rheostat

Answer 4

• adjusts light intensity
• also called light intensity knob

Question 5

base

Answer 5

• bottom part of frame
• contains light source

Question 6

light source

Answer 6

• found within the base
• illuminates the specimen

Question 7

ocular lenses

Answer 7

• the eyepieces provide upright virtual image
• 10X magnification

Question 8

revolving nosepiece

Answer 8

• supports objective lenses
• rotates to change objective lens

Question 9

slide clips

Answer 9

• holds slide in place on stage

Question 10

mechanical stage

Answer 10

• platform for slide to sit on

Question 11

mechanical stage adjustor knob

Answer 11

• moves stage forward, back, left, or right

Question 12

fine focus knob

Answer 12

• used for precision focusing

Question 13

coarse focus knob

Answer 13

• raises or lowers the stage

Question 14

objective lenses

Answer 14

• create real upside down image
• four strengths

Question 15

scanning lens

Answer 15

4x magnification

Question 16

low power lens

Answer 16

10x magnification

Question 17

high power lens

Answer 17

40x magnification

Question 18

oil immersion lens

Answer 18

100x magnification

Question 19

condenser

Answer 19

• lens below the stage
• concentrates light onto the specimen

Question 20

iris diaphragm

Answer 20

• adjusts contrast
• opens and closes to regulate the amount of light reaching the condenser

Question 21

cytology

Answer 21

study of cells

Question 22

histology

Answer 22

study of tissues

Question 23

gross anatomy

Answer 23

structures large enough to see with the naked eye

Question 24

Before using the microscope

Answer 24

• clean all lenses by carefully wiping them with lens paper.
• plug in the microscope
• make sure the rheostat is on its minimum position before turning on the light source

Question 25

finished working with the microscope

Answer 25

• remove the slide & wipe the oil off the immersion oil objective and the slide
• rotate the nosepiece to put the scanning lens in place and turn the coarse adjustment knob so that the stage is in its lowest position
• turn the rheostat to low before turning off the light source
• turn off the light source and remove the slide
• use the mechanical stage adjuster knob to make sure the stage arm is not sticking out beyond the stage
• unplug the power cord and carefully wrap it around the microscope and tuck in the plug so that there are no dangling cords;
• cover microscope (if cover available); andcarefully carry it to its proper storage cabinet.

Question 26

resolution

Answer 26

The capability of human eye or a device to distinguish finer details of an object

Question 27

resolution of human eye

Answer 27

0.14 mm/140 micrometer

Question 28

resolution of light microscope

Answer 28

0.0002 mm/0.2 micrometer

Question 29

field of view

Answer 29

area of the slide that is visible to you when looking through the microscope

Question 30

working distance

Answer 30

It is the distance between the front lens of the objective and the surface of the cover glass on the slide when the object is in sharp focus.

Question 31

high power

Answer 31

• lens closest to slide
• 400x total magnification
• smallest field of view
• largest image

Question 32

low power

Answer 32

• lens medium distance from slide
• 100x total magnification
• intermediate size field of view
• intermediate size image

Question 33

scanning power

Answer 33

• lens farthest from the slide
• 40x total magnification
• largest field of view
• smallest image

Question 34

Parfocal objectives

Answer 34

need little or no fine adjustment when moving from one to the other objective.

Question 35

cytoplasm

Answer 35

• semi-solid substance of cell
• location of organelles

Question 36

nucleus

Answer 36

• contains cell’s DNA

Question 37

plasma membrane

Answer 37

• outermost boundary of the cell