microscope

Question 1

What is Head of the microscope?

Answer 1

It is also called the body, it carries the optical parts in the upper part of the microscope.

Question 2

What is Base of microscope?

Answer 2

It acts as microscopes support. It also carriers the microscopic illuminators.

Question 3

WHat is Arms of the microscope?

Answer 3

This is the part connecting the base and to the head and the eyepiece tube to the
base of the microscope. It gives support to the head of the microscope and it also
used when carrying the microscope

Question 4

What is Eyepiece of the microscope

Answer 4

also known as the ocular. this is the part used to look through the microscope. Its
found at the top of the microscope. Its standard magnification is 10x. In some
microscopes such as binoculars , For monocular microscopes.

Question 5

What Is Objective lenses of the microscope?

Answer 5

These are the major lenses used for specimenvisualization. They have a
magnification power of 40x 100X.

Question 6

What is Eyepiece tube of the microscope?

Answer 6

its the eyepiece holder. It carries the eyepiece just above the objective lens

Question 7

What is Stage of the microscope?

Answer 7

This is the section on which the specimen is placed for viewing. They have stage
clips hold the specimen slides in place.

Question 8

What is The Adjustment knobs of the microscope?

Answer 8

These are knobs that are used to focus the microscope. There are two types of
adjustment knobs i.e fine adjustment knobs (used for sharp foucs ) and the coarse
adjustment knobs.

Question 9

What is Iris diaphragm of the microscope?

Answer 9

It is found under the stage of the microscope and its primary role is to control the amount of light that reaches the specimen.

Question 10

What IS Condenser of the microscope?

Answer 10

lens These are lenses that are used to collect and focus light from the illuminator
into the specimen.

Question 11

What is Microscopic illuminator of the microscope?

Answer 11

This is the microscopes light source, located at the base.

Question 12

What is Revolving nose piece of the microscope?

Answer 12

It holds the objective lenses

Question 13

Where is the Electron microscope used for?

Answer 13

It is used for studying cell details.

Question 14

There are two types about Electron microscope:

Answer 14

1. Transmission electron microscope (TEM) which used to study internal structure
   of the cell.
2. Scanning electron microscope (SEM) which used to study the surface of the cell.

Question 15

What the highest magnification power on Electron microscope?

Answer 15

1000000X.

Question 16

What is the Source of illumination of electron microscope?

Answer 16

electron beams.

Question 17

What the stain used of electron microscope?

Answer 17

heavy metals.

Question 18

What is the image can see when use dissecting microscope ?

Answer 18

is real not inverted. Specimen can be
viewed as a whole object whatever live or dead.

Question 19

What is dissecting microscope used?

Answer 19

used to view three dimensional objects and large
specimens such as insect.

Question 20

What like the image when see it by light microscope?

Answer 20

•The image created by this microscope is inverted and two
dimensional.

Question 21

what the specimen must be used in light microscope?

Answer 21

sectioned and stained.

Question 22

What is the most popular stain used to create contrast in the light microscope?

Answer 22

Hematoxylin and
Eosin (H&E stain).

Question 23

Hematoxylin stains the nucleus ….., while eosin

stains the cytoplasm …. .

Answer 23

1. Blue

2. Pink

Question 24

Based on illumination source, microscopy can be divided into two main categories:

Answer 24

1. light

2. Electron beams:

Question 25

light microscopy:

Answer 25

1. Compound light microscope
2. Phase contrast microscope
3. Dissecting microscope

Question 26

What is the purpose of simple staining?

Answer 26

to elucidate the
morphology and arrangement of bacterial cells.

Question 27

What is the most commonly
used basic stains of simple stain?

Answer 27

are methylene blue, crystal violet, and carbol fuchsin.

Question 28

Basic stains with a positively charged chromogen are

preferred,why?

Answer 28

because bacterial nucleic acids and certain cell wall components
carry a negative charge that strongly attracts and binds to the cationic
chromogen.

Question 29

In simple staining, the bacterial smear is stained with a single reagent, what produces?

Answer 29

which produces a distinctive contrast between the organism and its background.

Question 30

What the difference between gram-positive and gram-negative bacteria?

Answer 30

the chemical structure of the cell wall

Question 31

What is the Bacterial cell wall contains ?

Answer 31

negatively charged aggregates such as
the carboskyl-coo groups carboxyl

Question 32

Why used the stain
simple it is used for preliminary examination ?

Answer 32

to ensure the presence of bacteria in a specific sample.

Question 33

What are dyes used for preliminary examination to ensure the presence of Bacteria?

Answer 33

Methylene alkaline crystal violet, basic purple dye acid tinctures safranine, malachite green. acid fucsin and congo red.

Question 34

Where use Flaming Sterilization?

Answer 34

This is a standard technique for little metal or glass objects, but not for large ones.

Question 35

What damage is accompanied by process Gram stain?

Answer 35

This method has the most health and
environmental damages,
1.including bacterial contamination as a result of the
dyeing process in public places in the laboratory,
2. which helps spread
microbial contamination that may cause injury to workers in the laboratory.
3.Laboratory or its spread to sewage water when washing slides with running
water.
4. In addition, this technique results in chemical contamination resulting
from bacterial dyes that are thrown into sewage water,
5.in addition to
economic damage as a result of damage to places where the dyeing
process takes place with the continuous consumption of materials used in
dyeing.