Bacterial Cultivation/Culturing

Question 1

What is A microbial culture?

Answer 1

is a way of multiplying microorganisms under
controlled laboratory circumstances by allowing them to replicate in preset culture
media.

Question 2

Where is A microbial culture used for?

Answer 2

used to identify the type of organism, as well as its
abundance in the sample under investigation.

Question 3

To investigating their morphology and identity, what you do?

Answer 3

Bacterial isolation. bacterial properties, or cultivating bacteria, is the first stage .

Question 4

keeping stock cultures alive or dead.

Answer 4

Alive

Question 5

Why calculate the number of viable organisms ?

Answer 5

to see if you’re allergic to antibiotics.

to
develop antigens for use in the laboratory.

Question 6

bacteria must also be cultivated in vitro:

Answer 6

for certain genetic research and cell modifications

Question 7

Classification Culture Media based on consistency?

Answer 7

A.Liquid media.
B. Solid media.
C. Semi solid media

Question 8

Bacterial culture media can be classified in at least in three ways:

Answer 8

1. Consistency
2. Nutritional component
3. Functional use

Question 9

Where keep A.Liquid media?

Answer 9

test-tubes, bottles or flasks.

Question 10

what referred sometimes about Liquid media?

Answer 10

“broths” (e.g nutrient broth)

Question 11

In liquid medium,

bacteria grow ….. producing general …….

Answer 11

1. uniformly

2. turbidity

Question 12

Where are a liquid medium use ?

Answer 12

Mostly
used for inoculums preparation.

Question 13

Do you added agar in liquid medium?

Answer 13

No agar is added.

Question 14

What is An agar plate ? and what contain ? and what to use for?

Answer 14

is a Petri dish that contains a growth medium
(typically agar plus nutrients)
used to culture microorganisms.

Question 15

Do you added agar in the sold medium or agar plate ?

Answer 15

2% of agar is added.

Question 16

…. is the most commonly used solidifying agent.

Answer 16

Agar

Question 17

Classification Culture Media based on Nutritional Components:

Answer 17

A. Simple media.
B. Complex media.
C. Synthetic or chemically defined media

Question 18

What mean the colony?

Answer 18

single bacterium, supplied with right
nutrients, will multiply on the solid medium in a limited area to form a colony,
which is a mass of cells all descended from the original one.

Question 19

How obtained Pure cultures? what are use?

Answer 19

using variety of special techniques.

All
glassware, media and instruments must be sterilized i.e. aseptic techniques

Question 20

Give examples about agar that use in sold medium?

Answer 20

Examples include Nutrient agar and
Blood agar.

Question 21

What can you observe when use a sold medium?

Answer 21

Colony morphology, pigmentation, hemolysis can be appreciated

Question 22

Where do use the semi-solid medium?

Answer 22

can be used in fermentation studies, in determine bacterial motility, and in promoting an aerobic growth.

Question 23

Semi-solid media:

Answer 23

Such media are fairly soft

Question 24

Give examples of
Semi-solid media ?

Answer 24

(Hugh & Leifson’s oxidation fermentation)

Question 25

Do you added agar in the semi-solid medium?

Answer 25

0.5% agar is added.

Question 26

What the benefits of the semi-solid medium?

Answer 26

useful in demonstrating bacterial motility and separating motile from non-motile strains.

Question 27

What the natural of bacteria existing in An artificial culture media?

Answer 27

1. Water,
2. a supply of carbon
3. energy
4. a source of nitrogen,
5. trace elements,
6. some growth agents make up a
   culture media.
7. The medium’s pH must be adjusted correspondingly.

Question 28

An artificial culture medium must provide :

Answer 28

the environmental and nutritional
conditions

Question 29

cultivation on solid media is
another convenient way of …… in mixture.

Answer 29

separating bacteria

Question 30

basic requirement for obtaining a pure culture are ….

medium

Answer 30

solid

Question 31

In the laboratory bacteria are isolated and grown in pure culture in order to:

Answer 31

study the functions of a particular specie

Question 32

What is the most important feature of pure culture?

Answer 32

++pure culture is a population of cells or
growing in the absence of other species or types.

++a pure culture may originate from
a single cell or single organism

Question 33

What is Synthetic media/Chemically defined media? How prepared? Give examples?

Answer 33

∆Specially prepared media for
research purposes where the composition of every component is well known.
∆ It is
prepared from pure chemical substances.
∆ex: peptone water (1% peptone + 0.5%
NaCl in water)

Question 34

Give me examples about the simple media?and do you added agar?

Answer 34

ex: NB, NA. Nutrient

Broth consists of peptone, yeast extract and NaCl. When 2% of agar is added to Nutrient Broth it forms Nutrient