Micrometry

Question 1

2 ways of Identifying Microorganisms

Answer 1

Morphological & Genetics

Question 2

looking into the outer or physical appearance

ex. color, clumping, solitary, etc.

Answer 2

Morphological identity

Question 3

simply for confirmation after morphological, more accurate and extensive

Answer 3

Genetic Testing

Question 4

for quick answer (medtech → positive or negative in bacteria)

Answer 4

Technique 1: Estimated Size (ES)

Question 5

unit of measurement for ES

Answer 5

mm - millimeter

Question 6

What is ES?

Answer 6

Estimated Size

Question 7

Formula for ES

Answer 7

FOV / (Number of specimen) (cells across}

Question 8

more accurate, formulas are more complex, in the context of academia

Answer 8

Technique 2: Actual Size (AS)

Question 9

What is AS?

Answer 9

Actual Size

Question 10

unit of measurement for AS

Answer 10

um - micrometer/microns

Question 11

What is CF?

Answer 11

CALIBRATION FACTOR

Question 12

When is CF done? Before or after

Answer 12

done before the activity (calibrating)

Question 13

CF is used to calibrate the ocular micrometer ( ? value) using a stage micrometer ( ? value)

Answer 13

unknown - ocular micrometer
known - stage micrometer

Question 14

dimensions won’t change
has an unknown value
looks like a coin; circular

Answer 14

Ocular Micrometer

Question 15

separate from microscope; you put it in ocular lens; not standard in microscope

Answer 15

Ocular Micrometer

Question 16

for measuring

Answer 16

Ocular Micrometer

Question 17

dimensions change
has a known value (10 microns per space/division) glass and rectangular

Answer 17

Stage Micrometer

Question 18

like a glass slide; put on stage

Answer 18

Stage Micrometer

Question 19

for calibration

Answer 19

Stage Micrometer

Question 20

ES & AS

Both techniques are widely acceptable. T or F

Answer 20

TRUE