Microfilaria Flashcards
Give the Life Cycle of Microfilaria
> L3 Larva will be introduced by the vector through skin inoculation.
Inside the body, larva migrates to the tissue and becomes adult.
The adult worms may reside in the lymphatic, subcutaneous tissue or internal body cavities.
Adult female worms lay alive microfilariae in which it may be released in the body via blood meal by the arthropod vector.
L1 transforms until L3 in body of the blood sucking insects and repeat life cycle
What is the Specimen Requirements for Microfilaria?
> As Adult female gives birth to a larval embryo that appears into the blood 1 year after infection
The rhythmical appearance of microfilaria
> PERIODICITY
The Largest number of microfilaria appeared at night
> Nocturnal Periodic (9PM - 4AM)
The Largest number of microfilariae appeared at day
> Diurnal Periodic (10:15AM-2:15PM)
Constant levels day and night
> Non-periodic
Can be detected at day but high numbers in late afternoon or night
> Sub-periodic or Nocturnal Sub-Periodic
Enumerate the 6 Laboratory Diagnosis for Microfilaria
- Blood
- Scraping from infected nodule and skin snip
- Concentration technique
- Serological Test
- Opthalmologic examination using slit lamp
- Ultrasonography
Giemsa Stained or filtering of heparinized blood using nucleopore filter (Stage: Chronic inefction)
> Blood
Knot (for small amount of microfilaria) or 1mL blood immersed in 10mL 2% formalin solution to lyse red cells.
> Concentration technique
Filarial worms residing in eyes.
> Opthalmologic examination using slit lamp
Presence of filarial dance sign.
> Ultrasonography
Give the 3 Pathogenicity under Microfilaria
- W. bancrofti
- Loa Loa
- Onchocerva volvulus
Tissue swelling due to obstruction of lymph glands
> Elephantiasis/ “malabar leg”
Collection of fluid in the membrane surrounding the testicles causing the swelling in the scrotum
> Hydrocoele
