MICROCOCCACEAE, STAPHYLOCOCCI, STREPTOCOCCI Flashcards
GRAM+, CATALASE POSITIVE (VIGOROUS BUBBLING), NON-MOTILE, FACULTATIVE, MODIFIED OXIDASE Positive, FURAZOLIDONE RESISTANT, LYSOSTAPHIN Resistant, BACITRACIN Susceptible, GROWS IN 6.5% NACL
MICROCOCCUS
CATALASE-POSITIVE, NONMOTILE, FACULTATIVE ANAEROBES, GROWS IN 6.5% NACL, BACITRACIN RESISTAN FURAZOLIDONE AND LYSOSTAPHIN SENSITIVE
STAPHYLOCOCCI
THIS GENUS IS PART OF THE NORMAL ORAL FLORA AND IS RARELY ISOLATED FROM INFECTION. THE COLONIES ADHERE STRONGLY TO THE AGAR SURFACE CATALASE NEGATIVE, BACITRACIN, FURAZOLIDONE RESISTANT DOES NOT GROW IN 6.5% NACL:
Stomatococus
GRAM-POSITIVE, CATALASE(-), LAP POSITIVE, NON MOTILE, FACULTATIVE ANAEROBE:
Streptococci
(All are LAP +)
OPPORTUNISTIC PATHOGENS FOUND ONLY IN IMMUNOCOMPROMISED PERSONS
MICROCOCCI
ENTEROTOXINS A AND D
Occurs 2-6 hours of consumption of contaminated food
FOOD POISONING
BOILS, CARBUNCLES FURUNCLES AND FOLLICULTIS LUNCH BOX FOOD
S AUREUS
multisystem disease characterized by high fever, rash, hypotension, shock, desquamation of the hands and feet, and possible death
TSS TAMPON ASSOCIATED
EXTENSIVE EXFOLIATIVE DERMATITIS ____________________ CAUSED BY EPIDERMOLYTIC OR EXFOLIATIVE TOXIN
SCALDED SKIN SYNDROME (RITTER’S DISEASE)
MORTALITY RATE FOR RITTER’S IS HIGHER IN ADULTS (CHRONIC RENAL FAILURE/IMMUNOCOMPROMISED THAN CHILDREN
TOXIN A AND B
ENTEROTOXIN F
TSST-1
SECONDARY TO BACTEREMIA
OSTEOMYELITIS
SECONDARY TO INFLUENZA
STAPH PNEUMONIA
SECONDARY TO INFLUENZA
STAPH PNEUMONIA
MAJOR S. AUREUS VIRULENCE MARKER
COAGULASE
*HELPS THE BACTERIUM (SAU) AVOID PHAGOCYTOSIS:
PROTEIN A
ESSENTIAL FOR SPREADING OF INFECTION
HYALURONIDASE /DURAN REYNAl
CAUSES RBC HEMOLYSIS (SAU)
alpha and beta Hemolysin
IN SBA ROUND, SMOOTH AND WHITE OR PIGMENTED (YELLOW ORANGE, BETA HEMOLYTIC
S. AUREUS
FACILITATES SKIN COLONIZATION :
LIPASE
SAU grows in
GROWS IN MSA CNA. PEA
IS A PRESUMPTIVE TEST, SINCE S. EPIDERMIDIS AN ALSO FERMENT MANNITOL
MSA
RAPID TEST TO DETECT PROTEIN A IN S. AUREUS
Passive Hemaggulutination
NORMAL FLORA IN HUMANS AND ANIMALS, HOSPITAL ACQUIRED (NOSOCOMIAL), ASSOCIATED WITH CATHETERIZATION PROSTHETIC DEVICE IMPLANTS, AND IMMUNOSUPPRESSIVE THERAPY
CONS
LESS COMMON SPECIES of CoNS,
S. haemolyticus
S. lugdunensis
S. Schleiferi (non coagulans)
SAPROPHYTICUS AND S. EPIDERMIDIS ON SHEEP BLOOD AGAR, COLONIES ARE USUALLY
ROUND, SMOOTH, AND WHITE WITHOUT HEMOLYSIS
NOVOBIOCIN RESISTANT 5uG CONCENTRATION
S. SAPROPHYTICUS,
S. KLOOSII
S. XYLOSUS,
S COHNI,
BIOFILM-DELTA TOXIN (SLIME)
SIMILAR VIRULENCE FACTORS WITH S LUGDUNENSIS AND S. HAEMOLYTICUS
S. Epidermidis
ENDOGENOUS STRAIN: STERILE SITE, BY IMPLANTATION OF MEDICAL DEVICES (EG, SHUNTS, PROSTHETIC DEVICES
S. Epidermidis
ENDOGENOUS STRAIN STERILE URINARY TRACT, NOTABLY IN YOUNG, SEXUALLY ACTIVE FEMALES
S SAPROPHYTICUS
ENDOGENOUS STRAIN: UNCERTAIN, RARELY IMPLICATED IN INFECTIONS IMMUNOCOMPROMISED HOSTS
MICROCOCCUS
IMPETIGO
SAU, S. pyo, MRSA
PCR FROM CLINICAL SWABS (MEC A)
MRSA
MRSA, IT IS RECOMMENDED THAT POSITIVE NUCLEIC ACID-BASED TESTING BE UTILIZED AS A _________________ AND ____________CULTURE AND ANTIMICROBIAL SENSITIVITY TESTING IS RECOMMENDED
PRELIMINARY RESULT; CONFIRMATORY
Medium To Large (0.5-1.5 µm): Smooth. Entire, Slightly Raised, Low Convex,
Opaque: Most Colonies Pigmented Creamy Yellow,
Most Colonies Beta Hemolytic
S. ureus
SMALL TO MEDIUM: OPAQUE,
GRAY WHITE COLONIES,
MOST COLONIES NONHEMOLYTIC SLIME PRODUCING STRAINS ARE EXTREMELY STICKY AND ADHERE TO THE AGAR SURFACE
S EPIDERMIDIS
Small to medium (1-2 µm), opaque, convex, non-hemolytic wide variety of pigments (white, tan, yellow, orange, pink)
MICROCOCCUS
LARGE ENTIRE VERY GLOSSY, SMOOTH, OPAQUE, BUTYROUS, CONVEX USUALLY WHITE BUT COLONIES CAN BE YELLOW OR ORANGE
S. SAPROPHYTICUS
TUBE COAG POSITIVE:_______________
EVENTHOUGH
VARiaBLE RESULTS FOR ______________ STILL CONVIDERED POSitive
Sau, S. hyicus, S. INTERMEDIUS ANDS SCHLEIFERI
S.. HYICUS AND INTERMEDIUS
COMMON RESISTANCE MECHANISM FOR STAPHYLOCOCi
B. Lactamase
