Microbiota Flashcards
Microbiota
Community of commensalism, symbiotic, and pathogenic microorganisms found in a specific environment or niche
Microbiome
All of the genomes of microbiota within specific niche
Alpha diversity
Within individual/single sample
Beta diversity
Between individuals/ between samples
Factors that influence microbiota
Anatomy environment hygenine meds diet dietary supplement genetics age general health
Location in body- gut resp skin
How to study microbiome
Culture, identifies 1-2% of bacteria
Culture independent OMICS
Independent of ability to establish optimal growth conditions
Next generation sequencing
ID whole genomes
16s ribosomal rna sequencing
All have 16s gene,
Can get specific information
What causes acidification of vaginal environment
Lactic acid
What bacteria is most common in women
Lactobacillis
What influences the vaginal microbiome
Age, ethnicity, lifestyle, contraceptives, anti/probiotics
Hormonal milieu and pregnancy
Produced by host
Glycogen, a amylase, igg iga, antimicrobial peptides
Produced by lactobacillus
Cytolysins, LDH, hydrogen peroxide h2o2
