Microbiology Test

Question 1

Purpose of microbiology test

Answer 1

identification of causative agent in Meningitis

Question 2

Microorganisms must be recovered by growing it on the

Answer 2

appropriate culture medium

Question 3

24 hrs

Answer 3

Bacterial meningitis

Question 4

6 weeks

Answer 4

Tubercular meningitis

Question 5

CSF centrifuged at

Answer 5

1500 rpm for 15 minutes

Question 6

sediments is used for

Answer 6

Slides
Culture

Question 7

Confirmatory rather than diagnostic procedure

Answer 7

CSF Culture

Question 8

Detection of gram-positive, gram-negative bacteria

Answer 8

Gram stain

Question 9

For mycobacterium

Answer 9

Acid Fast Stain

Question 10

For Cryptococcus neoformans

Answer 10

India ink

Question 11

For other causative agents of meningitis

Answer 11

Latex Agglutination Test

Question 12

Bacterial Meningitis

Predominant cell :
Increase protein :
Glucose :
Lactate :

Answer 12

Neutrophil
Marked
Markedly decreased
>35 mg/dl

Question 13

Viral Meningitis

Predominant cell :
Increase protein :
Glucose :
Lactate :

Answer 13

Lymphocyte
Moderate
Normal
Normal

Question 14

Tubercular meningitis

Predominant cell :
Increase protein :
Glucose :
Lactate :

Answer 14

Lymphocyte, monocytes
Moderate to marked
Decreased
>25mg/dl

Question 15

Fungal meningitis

Answer 15

Lymphocyte, monocyte, eosinophil
Moderate to mark
Normal to decrease
>25mg/dl

Question 16

Routinely performed on CSF from all suspected cases of meningitis

Answer 16

Gram stain

Question 17

Values lies in the detection of

Answer 17

bacterial and fungal organism

Question 18

Highly concentrated specimen

Answer 18

Cytocentrifuge

Question 19

results as gram-positive

Answer 19

acid-fast bacilli

Question 20

Common causes of bacterial meningitis in infants

Answer 20

S. agalactiae
E. coli (K1 strain)
L. monocytogenes

Question 21

Common causes of bacterial meningitis in adult

Answer 21

N. meningitides
H. influenza (Serotype B) S. pneumoniae

Question 22

Common causes of bacterial meningitis in elderly

Answer 22

S. pneumoniae
N. meningitides
L. monocytogenes

Question 23

[India Ink Preparation]

Cryptococcus neoformans

Answer 23

Fungal meningitis

Question 24

[India Ink Preparation]

Cryptococcal meningitis

Answer 24

AIDS

Question 25

[India Ink Preparation lab findings] India ink

Answer 25

Thick encapsulated organism

Question 26

[India Ink Preparation lab findings] Gram stain

Answer 26

Starburst pattern

Question 27

[India Ink Preparation lab findings] Associated with

Answer 27

Increased eosinophil

Question 28

Diagnosis of meningitis caused by Gram-negative bacteria

Answer 28

Limulus lysate test

Question 29

Reacts with bacterial endotoxin of gram-negative bacteria

Answer 29

Limulus amebocyte

Question 30

Limulus lysate test reagent

Answer 30

Horseshoe crab

Question 31

Horseshoe crab came from

Answer 31

Limulus Polyphemus

Question 32

Limulus lysate test positive result

Answer 32

Caogulation within 1hr of incubation at 37 deg C

Question 33

Rapid tests

Answer 33

Latex agglutination ELISA Bacterial antigen test

Question 34

Performed to detect the presence of Neurosyphilis

Answer 34

Serologic testing

Question 35

Procedure recommended by the CDC to diagnose Neurosyphilis

Answer 35

VDRL

Question 36

More sensitive than the VDRL Prevent contamination with blood because the FTA-ABS remains positive in the serum of treated cases of syphilis

Answer 36

FTA-ABS