Microbiology Test Flashcards
Purpose of microbiology test
identification of causative agent in Meningitis
Microorganisms must be recovered by growing it on the
appropriate culture medium
24 hrs
Bacterial meningitis
6 weeks
Tubercular meningitis
CSF centrifuged at
1500 rpm for 15 minutes
sediments is used for
Slides
Culture
Confirmatory rather than diagnostic procedure
CSF Culture
Detection of gram-positive, gram-negative bacteria
Gram stain
For mycobacterium
Acid Fast Stain
For Cryptococcus neoformans
India ink
For other causative agents of meningitis
Latex Agglutination Test
Bacterial Meningitis
Predominant cell :
Increase protein :
Glucose :
Lactate :
Neutrophil
Marked
Markedly decreased
>35 mg/dl
Viral Meningitis
Predominant cell :
Increase protein :
Glucose :
Lactate :
Lymphocyte
Moderate
Normal
Normal
Tubercular meningitis
Predominant cell :
Increase protein :
Glucose :
Lactate :
Lymphocyte, monocytes
Moderate to marked
Decreased
>25mg/dl
Fungal meningitis
Lymphocyte, monocyte, eosinophil
Moderate to mark
Normal to decrease
>25mg/dl