Microbiology Test Flashcards

1
Q

Purpose of microbiology test

A

identification of causative agent in Meningitis

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2
Q

Microorganisms must be recovered by growing it on the

A

appropriate culture medium

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3
Q

24 hrs

A

Bacterial meningitis

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4
Q

6 weeks

A

Tubercular meningitis

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5
Q

CSF centrifuged at

A

1500 rpm for 15 minutes

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6
Q

sediments is used for

A

Slides
Culture

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7
Q

Confirmatory rather than diagnostic procedure

A

CSF Culture

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8
Q

Detection of gram-positive, gram-negative bacteria

A

Gram stain

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9
Q

For mycobacterium

A

Acid Fast Stain

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10
Q

For Cryptococcus neoformans

A

India ink

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11
Q

For other causative agents of meningitis

A

Latex Agglutination Test

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12
Q

Bacterial Meningitis

Predominant cell :
Increase protein :
Glucose :
Lactate :

A

Neutrophil
Marked
Markedly decreased
>35 mg/dl

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13
Q

Viral Meningitis

Predominant cell :
Increase protein :
Glucose :
Lactate :

A

Lymphocyte
Moderate
Normal
Normal

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14
Q

Tubercular meningitis

Predominant cell :
Increase protein :
Glucose :
Lactate :

A

Lymphocyte, monocytes
Moderate to marked
Decreased
>25mg/dl

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15
Q

Fungal meningitis

A

Lymphocyte, monocyte, eosinophil
Moderate to mark
Normal to decrease
>25mg/dl

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16
Q

Routinely performed on CSF from all suspected cases of meningitis

A

Gram stain

17
Q

Values lies in the detection of

A

bacterial and fungal organism

18
Q

Highly concentrated specimen

A

Cytocentrifuge

19
Q

results as gram-positive

A

acid-fast bacilli

20
Q

Common causes of bacterial meningitis in infants

A

S. agalactiae
E. coli (K1 strain)
L. monocytogenes

21
Q

Common causes of bacterial meningitis in adult

A

N. meningitides
H. influenza (Serotype B) S. pneumoniae

22
Q

Common causes of bacterial meningitis in elderly

A

S. pneumoniae
N. meningitides
L. monocytogenes

23
Q

[India Ink Preparation]

Cryptococcus neoformans

A

Fungal meningitis

24
Q

[India Ink Preparation]

Cryptococcal meningitis

A

AIDS

25
Q

[India Ink Preparation lab findings]

India ink

A

Thick encapsulated organism

26
Q

[India Ink Preparation lab findings]

Gram stain

A

Starburst pattern

27
Q

[India Ink Preparation lab findings]

Associated with

A

Increased eosinophil

28
Q

Diagnosis of meningitis caused by Gram-negative bacteria

A

Limulus lysate test

29
Q

Reacts with bacterial endotoxin of gram-negative bacteria

A

Limulus amebocyte

30
Q

Limulus lysate test reagent

A

Horseshoe crab

31
Q

Horseshoe crab came from

A

Limulus Polyphemus

32
Q

Limulus lysate test positive result

A

Caogulation within 1hr of incubation at 37 deg C

33
Q

Rapid tests

A

Latex agglutination
ELISA
Bacterial antigen test

34
Q

Performed to detect the presence of Neurosyphilis

A

Serologic testing

35
Q

Procedure recommended by the CDC to diagnose Neurosyphilis

A

VDRL

36
Q

More sensitive than the VDRL

Prevent contamination with blood because the FTA-ABS remains positive in
the serum of treated cases of syphilis

A

FTA-ABS