Microbiology QS

Question 1

Appropriate clinical specimen to take on child for cariogenic organisms?

Answer 1

General salivary swab / tooth surface swab

• young child not cooperative

Question 2

What methods can be used to isolate bacteria?

Answer 2

Next generation sequencing

Microbiome analysis of 16s rDNA ribosomes

Question 3

What specimen may be taken for someone with gingivitis / periodontitis?

Answer 3

Subgingival plaque biofilm

• paper point from gingival crevice at site of inflammation

Question 4

What methods can be used to cultivate specimen from gingival crevice?

Answer 4

NGS

PCR

Selective agar followed by sensitivity testing

Question 5

What MOs are associated with denture stomatitis?

Answer 5

Candida albicans

Candida glabrata

Question 6

What sample can be taken from someone with denture stomatitis?

Answer 6

Oral rinse / oral swab

Question 7

What would be the reasoning to test the culture of denture stomatitis?

Answer 7

Candida glabrata is resistant to azole antifungals, so they would not work