Microbiology Lab Practical 01

Question 1

Who is Antone Van Leeuwenhoek

Answer 1

He is the founder of microbiology, invented the microscope.

Question 2

Who is Robert Koch

Answer 2

He is a German doctor who identified the bacterium that caused tuberculosis. Developed K-streak

Question 3

K-streak technique

Answer 3

used to get a pure culture of bacteria

Question 4

Nomenclature

Answer 4

First part: italicized and capitalized (generic name)
Second part: uncapitalized and specie name

Question 5

Bacillus

Answer 5

Rod shaped bacteria, gram positive, facultative anaerobe

Question 6

Aerobes

Answer 6

Bacteria that require oxygen to grow

Question 7

Facultative Anaerobes

Answer 7

can live with or without oxygen

Question 8

Obligate Anaerobes

Answer 8

can’t live with oxygen

Question 9

Clostridium

Answer 9

Found in soil and digestive system
Botulinum: food poisoning (toxin: botox)
diffficile: C-diff
Tetani: tetanus

Question 10

Saprophyte

Answer 10

An organism that feeds on dead or decaying matter

Question 11

Catalase Test

Answer 11

used to identify organisms that produce the enzyme catalase and breaks down peroxide

Question 12

Simple Stain

Answer 12

nonspecific, single dye, determines shape, size arrangement. reads with all microbes

Question 13

Differential Stain

Answer 13

Multiple dyes that help identify microbes, organisms react in different ways

Question 14

Simple stain basic dyes

Answer 14

positively charged, binds with negative cells ex methylene blue

Question 15

Simple stain acidic dyes

Answer 15

negatively charges, bind to positive. Help with background

Question 16

staphylococcus

Answer 16

Gram-positive, catalase positive, facultatively anaerobic
aureus; golden staph (MRSA), produce capsule that avoids immune system, pigment staphyloxanthin

Question 17

Nutrient broth/ agar

Answer 17

general, complex, supportive media

Question 18

differential media

Answer 18

allows growth of several types of microbes and displays visible differences among those microbes because of change in media

Question 19

Selective media

Answer 19

suppress unwanted microbes and encourage desired microbes

Question 20

defined media

Answer 20

media prepared with a specific chemical composition