Microbiology Lab Ex 2

Question 1

What is the purpose of heat fixing?

Answer 1

To allow the smear to adhere to the slide, so it wont easily get washed off during the staining process

Question 2

What was the specific method used in gram stain?

Answer 2

Hucker’s Method

Question 3

What was the specific technique used in Acid fast staining?

Answer 3

Cold Kinyoun Technique

Question 4

Name of main stain used in gram staining?

Answer 4

Gentian violet

Question 5

Used to decolorize the smear in gram staining

Answer 5

Acetone alcohol

Question 6

Counter stain used in gram staining

Answer 6

Safranin dye

Question 7

Main stain used in acid fast stain

Answer 7

Carbol fuschin

Question 8

Decolorizer of smear in acid fast staining?

Answer 8

Acidic alcohol

Question 9

Counter stain used in acid fast staining?

Answer 9

Methylene Blue

Question 10

General rules on gram staining

Answer 10

All cocci are gram + except Neisseria and Moraxella; All bacilli are gram - except Acid fast bacteria, spore formers, and Mycobacterium species; All spirals are gram -

Question 11

What structure is resp for the acid-fastness of certain bacteria

Answer 11

Presence pf mycolic acid

Question 12

Purpose of air drying?

Answer 12

To preserve the morphology of the organisms