Microbiology Lab Ex 1

Question 1

What was the stain used for S. pneumoniae and K. Pneumoniae?

Answer 1

Anthony stain

Question 2

3 basic morphologic types of bacteria

Answer 2

Cocci, Bacilli, Spiral

Question 3

Subtypes of Cocci, give example for each

Answer 3

Staphylococci (S. aureus), Streptococci (S. pyogenes), Diplococci (S. pneumoniae), Tetrad (Gaffkya tetragena), Sarcina (S. lutea)

Question 4

Subtypes of Bacilli, give example for each

Answer 4

Streptobacilli (Bacillus subtilis), Diplobacilli (Mycobacterium tuberculosis), Coccobacilli (Escherichia coli), Vibrio (Vibrio cholerae)

Question 5

Subtypes of Spiral bacteria

Answer 5

Spirochetes, Borrelia, Leptospira

Question 6

How are bacteria classified?

Answer 6

Bacteria are prokaryotes

Question 7

What makes up the bacterial cell envelope?

Answer 7

Made up of peptidoglycan

Question 8

What was the stain used in Treponema pallidum?

Answer 8

Fontana Tribondeau stain

Question 9

Metachromatic (volutin) granules are made of?

Answer 9

Inorganic phosphate

Question 10

What 2 genera usu produce metachromatic granules?

Answer 10

Mycobacterium, Corynebacterium

Question 11

Function of flagella

Answer 11

Used in locomotion or motility

Question 12

Flagellar types based on distribution

Answer 12

Monotrichous, Lophotrichous, Amphitrichous, Peritrichous, Atrichous

Question 13

2 major genera where spores are demonstrated?

Answer 13

Bacilli and Clostridium

Question 14

Significance of spores

Answer 14

Enables the bacteria to resist dessication and heat

Question 15

Significance of presence of capsule

Answer 15

Help resist phagocytosis