Microbiology- bacteria Flashcards
What colour do gram negative bacteria appear under the microscope?
pink
What colour do gram positive bacteria appear under the microscope?
purple
Describe blood agar
contains sheeps/horse blood
Describe chocolate agar
Contains blood agar heated to 80 degrees c for 5 minutes. This releases some of the nutrients into the agar to make it easier to grow certain organisms that do not grow easily.
What does CLED agar stand for?
cysteine lactose electrolyte deficient
What colour is a lactose fermenting gram negative bacilli on CLED agar?
yellow
What colour is non-lactose fermenting gram negative bacilli on CLED agar?
blue
What type of bacteria is MacConkey agar primarily designed to grow?
gram negative bacilli
What does MacConkey agar contain that inhibits gram positive bacteria?
bile salts
Does MacConkey agar contain lactose?
yes
What colour are lactose fermenters on MacConkey agar?
pink
What colour are non-lactose fermenters on MacConkey agar?
yellow/colourless
What type of agar would you use to grow Neisseria spp. ?
gonococcus agar
What type of agar would you use to isolate salmonella spp. or shigella spp. ?
XLD agar
How do colonies of salmonella and shigella look on XLD agar?
salmonella- red with black centres
shigella- red
What type of agar is used to culture fungi?
Sabouraud’s agar