Microbiology- bacteria Flashcards

1
Q

What colour do gram negative bacteria appear under the microscope?

A

pink

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2
Q

What colour do gram positive bacteria appear under the microscope?

A

purple

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3
Q

Describe blood agar

A

contains sheeps/horse blood

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4
Q

Describe chocolate agar

A

Contains blood agar heated to 80 degrees c for 5 minutes. This releases some of the nutrients into the agar to make it easier to grow certain organisms that do not grow easily.

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5
Q

What does CLED agar stand for?

A

cysteine lactose electrolyte deficient

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6
Q

What colour is a lactose fermenting gram negative bacilli on CLED agar?

A

yellow

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7
Q

What colour is non-lactose fermenting gram negative bacilli on CLED agar?

A

blue

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8
Q

What type of bacteria is MacConkey agar primarily designed to grow?

A

gram negative bacilli

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9
Q

What does MacConkey agar contain that inhibits gram positive bacteria?

A

bile salts

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10
Q

Does MacConkey agar contain lactose?

A

yes

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11
Q

What colour are lactose fermenters on MacConkey agar?

A

pink

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12
Q

What colour are non-lactose fermenters on MacConkey agar?

A

yellow/colourless

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13
Q

What type of agar would you use to grow Neisseria spp. ?

A

gonococcus agar

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14
Q

What type of agar would you use to isolate salmonella spp. or shigella spp. ?

A

XLD agar

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15
Q

How do colonies of salmonella and shigella look on XLD agar?

A

salmonella- red with black centres

shigella- red

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16
Q

What type of agar is used to culture fungi?

A

Sabouraud’s agar

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17
Q

Which genus of bacteria is gram positive cocci and arranged in clusters?

A

Staphylococcus

18
Q

What genus of gram positive cocci is found in chains?

A

Streptococcus

19
Q

For what genus of gram positive cocci would you carry out haemolysis on blood agar to classify further?

A

Streptococcus

20
Q

Describe the arrangement of Staphylococcus

A

clusters

21
Q

Describe the arrangement of Streptococcus

A

chains

22
Q

How are beta haemolytic strep further classified?

A

by antigenic group

23
Q

Are viridan’s strep alpha or beta haemolytic?

A

alpha

24
Q

What type of alpha haemolytic strep is sensitive to the optochin test?

A

S. pneumoniae

25
Q

Which Staphylococcal species will produce a positive coagulase test?

A

S. aureus

26
Q

Describe the disease causing potential of Staphylococcus aureus compared to Staphylococcus epidermidis ?

A

Disease causing potential of S. aureus is high

Disease causing potential of S. epidermidis is low

27
Q

What colour are S. aureus colonies on blood agar ?

A

gold (aureus is latin for gold)

28
Q

Which out of S. aureus and S. epidermidis displays coagulase activity?

A

S. aureus

29
Q

Describe the colonies of S. epidermidis

A

White/not pigemented

30
Q

coagulase-negative staphylococci are a common cause of what infection?

A

line infection

31
Q

What does a positive coagulase test look like?

A

clot formation in test tube

32
Q

Describe what beta haemolysis looks like on blood agar

A

clearing of agar round colonies

33
Q

describe what alpha haemolysis looks like on blood agar?

A

greening of agar round colonies

34
Q

How do you test alpha haemolytic strep for S.pneumoniae?

A

optochin test

35
Q

What can Streptococcus pneumoniae cause?

A

lobar pneumoniae and meningitis

36
Q

What further grouping can we do to beta haemolytic streptococci?

A

Lancefield grouping

37
Q

What antigen do enterococci have?

A

D

38
Q

What antigens on beta haemolytic streptococci can cause skin infection and tonsilitis?

A

A, C and G

39
Q

What can beta haemolytic strep with B antigen cause?

A

neonatal sepsis and meningitis

40
Q

What type of haemolysis is it when there is a clear zone around colonies?

A

beta