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Microbiology Flashcards

1
Q

Difference between eukaryotic and prokaryotic transcription and translation

A

Eukaryotes

Transcription (nucleus) and translation (cytoplasm) occur separately

  • Introns splicing post-transcription

Prokaryotes

Both processes take place in the cytoplasm stimultaneously

  • Does not contain introns
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2
Q

Difference between eukaryotic and prokaryotic composition of ribosomes

A

Eukaryotes

  • 40S and 60S subunits (larger) to yield 80S

Prokaryotes

  • 30S and 50S subunits to yield 70S
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3
Q

The two prokaryotic domains

A

Archaea

Bacteria

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4
Q

Bacilli vs. cocci vs. spirilla

A

Bacilli

  • Long and rod-shaped
  • Strepts and staphyls

Cocci

  • Spherical
  • Flagellate rods (Salmonella typhi)
  • Spore-former (Clastridium botulism)

Spirilla

  • Helical/”spiral-shaped” (H. pylori)
  • Vibrios (vibrio cholerae)
  • Spirochaetes (Treponema pallidum)
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5
Q

Bacterial cell wall

A

CW is peptidoglycan

  • Gram +: thick peptidoglycan (purple)
    • Endospore: formed under harsh environment (mostly Gram +)
  • Gram -: thin peptidoglycan with a outer membrane (pink); lipopolysaccharide
    • The outer membrane may cause Gram - bacteria harder to treat with antibotics

(Fungal CW is chitin)

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6
Q

Flagellum

A

Facilitates bacterial motion

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7
Q

Most common form of bacterial reproduction

A

Binary fission

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8
Q

Bacterial gene transfer

A

Transformation

Acquiring DNA from environment (plasmid = small piece of circular DNA)

  • Usually occur during crowding or starvation (aging colony); transformation can be triggered by starvation

Transduction

Being injected DNA by a bacteriophage

  • May be detrimental to bacteria, but may be advantagous to the virus

Conjugation

Direct DNA transfer via contact bw bacterial cells with sex pilus

  • An adaptive mechanism when resources are scarce
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9
Q

Transposon

A

A DNA sequence with the ability to excise itself from the genome and move to another location

  • In both prokaryotes and eukaryotes, transposons are located in the chromosomes
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10
Q

Production of food of bacteria

A

Autotrophs

Can produce their own food

  • Photoautotrophs & chemoautotrophs

Heterotrophs

Consume external organic compounds

  • Photoheterotrophs & chemoheterotrophs
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11
Q

Bacterial ecology

A

Obligate anaerobes

Die in presence of oxygen

Facultative anaerobes

Can survive with or without oxygen

  • E. coli

Thermophile

High temp

Mesophile

Medium temp

Psychrophile

Low temp

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12
Q

Symbiosis

A

Symbiosis: relationship between 2 species

  • Mutualism
  • Commensalism
  • Parasitism
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13
Q

Characteristics of a virus

A

Virus can only replicate inside a host cell

(Virus are not living organisms)

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14
Q

Lytic cycle

A
  • Viruses that replicate by the lytic cycle, killing their host cells, are called virulent
  • If then virus does not lyse the cell, it becomes integrated into the bacterial genome in a less harmful form (provirus/prophage), lying dormant for one or more generations as a part of the lysogenic cycle (latent infection)
  • Ex. HIV (also a retrovirus that infects helper T cells)
  • Either spontaneously or environmental circumstances, the provirus cell enter a lytic cycle
  • Superinfection: cells containing proviruses are normally resistant to further infection by similar phages
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15
Q

Atypical viruses

A

Prion

  • Disease-causing protein
  • Made up of only protein, no DNA
  • Mad cow disease

Viroid

  • Disease-causing molecule (mainly in plants)
  • Made up of only a single-stranded RNA, no protein
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16
Q

Epidemiology of bacteria

A

Pathogen transmission from animals (zoonoses); from hosptial (nosocomial)

  • Normal flora in large intestine help sythesize vitamin K and B groups
17
Q

Pathogen mechanisms

A
  • Bacteria cause diseases due to toxins
  • Exotoxins: secreated by bacteria
  • Endotoxins: part of CW of Gram - bacteria
  • In small amounts –> inflammatory repsonse
  • In large amounts –> DIC (clotting), sepsis, and fatal septic shock
18
Q

Controlling infection

A

Physical method

  • Heat
  • Autoclaving achieves sterility
  • Pasteurization reduces microbial number
  • Radiation

Chemical method

  • Mostly denatures proteins
  • Antiseptic: on living tissues
  • Disinfectants: on non-living
  • Alcohol, I, Cl, H2O2, etc.
19
Q

Gram +, spherical

A
  • Wide range of infections on skin
  • Streptococci can cause pneumonia and strep throat
20
Q

Gram -, coccobacilli

A
  • Short rods that look like cocci
  • Bordatella causes pertussis (whooping cough)
21
Q

Gram +, rod-shaped

A

Clostridium (anaerobic) caused

  • Tetanus (muscle spasms): from rusty nails
  • Botulism (muscle weakness): from food poisoning
  • Gas gangrene: gas produced in necrotic tissues due to poor blood supply

Mycobacterium caused

  • Tuberculosis: lung infections
  • Leprosy: skin lesion, loss of feeling in extremities

Bacillus caused

  • Anthrax (skin lesion)
  • Food poisoning
22
Q

Gram -, rod-shaped

A
  • Indicators of pollution bc they are found in the gut or feces
  • Cause a variety of infections of the GI tract
  • E.g. Salmonella, E. coli, Shigella
23
Q

Gram -, spirals

A

Vibrios

  • Vibrio cholerae causes cholera: vomitting, diarrhea due to contaminated food and water

Spirilla

  • Helicobacter pylori causes gastric ulcersm chronic gastritis, linked to stomach cancer
24
Q

Spirochaetes

A

Borrelia

  • Causes Lyme disease (transmitted by ticks)

Treponema

  • Causes syphilis, STD
25
Antibacterial drugs
- Selective toxicity * Inhibit peptidoglycan synthesis and damage CW * Effective against Gram + bacteria * Developed my Alexander Fleming * E.g. penicillin (reactive 4C ring irreversibly bind to bacteria) - Interferes with protein synthesis * E.g. erythromycin (broad spectrum) - Blocks DNA, RNA synthesis by mimicking enzyme substrates * E.g. sulfa drugs
26
Protozoans
* Usually found in water and moist area * Single-cell eukaryotes that can move with flagella via amoeboid motion * **Traphozoite stage**: protozans are absorbing nutrients from the host * **Cyst stage**: tough survival stage that can be transmitted and cause diseases * Photosynthetic: *Euglena* * Predators: *Paramecium, Ameoba*
27
Protozan diseases
**Malaria** * Caused by *plasmodium falciparum* * Transmitted by mosquitos * Sickle cell anemia patients are resistant to malaria * Infects RBC of humans * Yellow skin, fever, nausea, coma **Sleeping sickness** * Caused by *trypanosoma gambiense* (African trypanasomiasis) * Cross blood-brain barrier * Transmitted by Tse Tse fly * Neurological disorder **Toxoplasma** * Usually no symptoms and untreated * Fetus can be infected if patient is preganant * Leading to abortion or neurological impairment in fetus * Associated with cats Protozan diseases of the intestine include *amebiasis* (caused by E. histolytica), *giardiasis* (caused by Giardia lamblia) and *cryptosporidiosis* (caused by Cryptosporidium)
28
Fungi
* Can be single (yeast) or multi-celled (mold) * Anti-fungal drugs target plasma membrane of fungi * Polyene or azoles * CW contains chitin
29
Fungal diseases
* Most common on the skin * Dermatophytes break down keratin in skin * Ringworm occurs in various areas of the body * Caused by *Epidermophyton* and *Trichophyton* * Yeast *Candida* can infect mouth, intestine or vagina * Esp. seen in immunosuppressed patients
30
Parasitic worms
* **Helminths**: via food or water * **Flatworms** (flukes, tape worms) and **roundworms** (hookworm, pinworm) * **Trichinella** aquired from undercooked pork and forms cyst in the muscle * **Schistosoma** and **hookworms** enter body via skin * **Tapeworm** from undercooked food * **Borrelia burgdorferi** is a tick borne parasite and causes Lyme disease
31
Viral diseases
* Influenza virus * Herpes, measles, rubella * Varicella (chicken pox) can recur later as shingles * HPV causes warts * Hepatitis A, B, C * Rabies, polio, yellow fever * HIV (single-stranded RNA)
32
Antiviral drugs
* Acyclovir for herpes * AZT reverse transcriptaase inhibitors for HIV * Resembles nucleotides and blocks enzymes for DNA synthesis
33
The nitrogen cycle
* Bacteria fix free *nitrogen* gas into *nitrates* and *nitrites* that are useable by plants * Plants produce *ammonia* and *ammonium ions* that are broken down by bacteria back into *nitrite* and *nitrate* * **Nitrogen fixation**: atmospheric N (N2) –\> ammonia (NH3) * **Nitrification**: oxidation of ammonia or ammonium –\> nitrile (ox) –\> nitrate * **Ammonification**: decomposition of organic nitrogen into ammonia * **Denitrification**: nitrate reduced to produce N2
34
Growth phases of bacteria
1. Stationary stage is characterized by both equal growth and death rates (0 growth) 2. Lag phase occurs right after inoculation of bacteria and before growth begins 3. Exponential phase is characterized by rapid doubling of bacteria population; growth rate is constant 4. Death stage is opposite of exponential stage
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PCAT Biology (5 decks)

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