Microbiology Flashcards
E. Coli
Gram -
rod
singles, large clumps
Shigella sonnei
Gram -
rod
singles, pairs, chains
Klebsiella pneumoniae
Gram -
rod
singles, short chains
Haemophilus influenzae
Gram -
coccobacilli
Pseudomonas aeruginosa
Gram -
rod
Neisseria gonorrgoeae
Gram -
diplococci
pairs
Moraxella catarrhalis
Gram -
diplococci
Treponema pallidum
Gram -
spiral
Streptococcus pyogenes
Gram +
cocci
Streptococcus pneumoniae
Gram +
diplococci
Staphylococcus aureus
Gram +
cocci
Bacillus anthracis
Gram +
rod
Clostridium tetani
Gram +
rod
Corynebacterium diphtheria
Gram +
rod
palisade
Gram Stain Procedure
Crystal Violet binds to cell walls
Iodine fixes CV
Decolorizer (Alcohol) removes CV from Gram - only
Counterstain (safranin) to color gram -
Gram Positive components
Lots of peptidoglycan,
Teichoic and Lipoteichoic acids form cell wall above cell membrane
Gram negative components
LPS (pathogenic lipopolysaccharides), Outer memb, periplasma w small peptidoglycan, and inner membrane
Blood Agar
heated sheeps blood, non selective but can be differential (types of hemolysis)
PEA Agar
Gram + selective
MacConkey Agar
Gram - selective
Staph Aureus on MSA plate
Aureus (gold) ferments mannitol of MSA and turns yellow
Serratia marcescens
At low temps, will grow RED
Staphylococcus aureus
Golden growth
Prok vs Euk cell (replication, components)
Prok- 50S and 30S has cell wall, no membrane bound organelles, single supercoiled chromosome, resp happens through membrane, asexual binary fission
Euk-60S and 40S, organelles, nucleus linear diploid chromosomes,