Microbiology Flashcards
1
Q
What is MacConkey Agar selective/differential for?
A
- Selective for Gram-Negative Bacteria (bile salts)
- Differential for Lactose fermentation (turns pink)
2
Q
What is Mannitol Salt Agar (MSA) selective/differential for?
A
- Selective for Staphylococci (salt)
- Differential for mannitol fermentation (pathogenic, turns yellow)
3
Q
What does Triple Sugar Iron (TSI) test for?
A
- Glucose fermentation (turns butt yellow, A)
- Lactose/Sucrose fermentation (turns slant yellow, A)
- Sulfur reduction (black precipitate)
4
Q
What does MIO test for?
A
- Motility- grow away from stab line
- Ornithine Decarboxylase (ODC)- turns medium purple
- Indole- presence of tryptophanase, turns red with Kovac’s
5
Q
What does citrate test for?
A
Bacterial expression of citrate permease (increases pH and turns blue)
6
Q
What does urease test for?
A
If bacteria can hydrolyze urea (increase pH, turns pink)
7
Q
What is a Sabouraud plate selective for?
A
Fungi
