Microbiology Flashcards

0
Q

Microaerophilic

A

Reduced oxygen

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1
Q

Facultative anaerobes

A

Prefers oxygen

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2
Q

What specific requirements do bacteria have

A

Temperature, ph, oxygen, nutrition

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3
Q

Mesophiles

A

Require a temperature of 24-40 degrees Celsius

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4
Q

Psychrophiles

A

Require a temperature less than 24

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5
Q

Thermophiles

A

Require a temperature greater than 40

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6
Q

What are the cell shapes

A

Cocci, bacilli, and spirals

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7
Q

What are the cell arrangements

A

Chains
Clusters
Pairs

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8
Q

What are spores resistant to?

A

Heat, radiation, and chemicals

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9
Q

What are the 4 phases of bacterial growth?

A

Initial
Exponential
Stationary
Final

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10
Q

Define transport media

A

Kept alive but not encouraged to grow

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11
Q

Define enriched media

A

Fastidious microbes

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12
Q

Selective media

A

Only grow the bacteria you want

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13
Q

Enrichment media

A

Grow a certain type of bacteria

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14
Q

Alpha hemolysis

A

Partial

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15
Q

Beta hemolysis

A

Complete

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16
Q

Gamma hemolysis

A

Can’t identify

17
Q

Delta hemolysis

A

Shows alpha and beta

18
Q

What is macconkey agar

A

Lactose fermenting enterobacteria
Selective and differential
Grow as pinkish red colonies

19
Q

What does thioglycollate broth culture

A

Anaerobic bacteria

20
Q

Bismuth sulfate agar

A

Suppresses Caliform and allows salmonella to grow

21
Q

Dermatophyte test media

A

Terns red in the presence of dermatophytes

22
Q

What are he 3 bacterial sample collections

A

Aspiration
Swab
Imprint

23
Q

Gram stain

A

Categorizes bacteria to gram positive or gram negative

24
What are the 4 steps to gram staining
Primary stain Mordant Decolorizer Counterstain
25
Gram positive color is..
Purple
26
Gram negative color is..
Red
27
KOH testing
Gram negative will develops a mucous appearance
28
Ziehl neelsen stain
Detects acid fast organisms
29
What colony characteristics do you look for?
Size, pigment, density, elevation, form, texture, Oder, hemolysis
30
What is motility media?
Used if organisms are nonmotile
31
What is a catalase test?
Performed on gram positive cocci and small gram positive bacilli If bubbles are seen- positive
32
What is the coagulase test?
Performed of catalase positive and gram positive cocci | If clots are seen it is positive
33
What is oxidase test?
If you see a purple color change within a minute it is positive
34
What is the California mastitis test
Tests for infected milk
35
Mycology means
Fungus
36
Anthropophilic
Lives on people
37
Zoophilic
Lives on animals
38
Geophilic
Lives in the environment
39
What color is a positive woods lamp?
Apple green
40
Is pasturella gram negative or gram positive?
Gram negative
41
Is bactericides gram negative or positive?
Gram negative