Microbiology Flashcards

0
Q

Microaerophilic

A

Reduced oxygen

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1
Q

Facultative anaerobes

A

Prefers oxygen

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2
Q

What specific requirements do bacteria have

A

Temperature, ph, oxygen, nutrition

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3
Q

Mesophiles

A

Require a temperature of 24-40 degrees Celsius

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4
Q

Psychrophiles

A

Require a temperature less than 24

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5
Q

Thermophiles

A

Require a temperature greater than 40

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6
Q

What are the cell shapes

A

Cocci, bacilli, and spirals

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7
Q

What are the cell arrangements

A

Chains
Clusters
Pairs

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8
Q

What are spores resistant to?

A

Heat, radiation, and chemicals

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9
Q

What are the 4 phases of bacterial growth?

A

Initial
Exponential
Stationary
Final

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10
Q

Define transport media

A

Kept alive but not encouraged to grow

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11
Q

Define enriched media

A

Fastidious microbes

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12
Q

Selective media

A

Only grow the bacteria you want

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13
Q

Enrichment media

A

Grow a certain type of bacteria

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14
Q

Alpha hemolysis

A

Partial

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15
Q

Beta hemolysis

A

Complete

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16
Q

Gamma hemolysis

A

Can’t identify

17
Q

Delta hemolysis

A

Shows alpha and beta

18
Q

What is macconkey agar

A

Lactose fermenting enterobacteria
Selective and differential
Grow as pinkish red colonies

19
Q

What does thioglycollate broth culture

A

Anaerobic bacteria

20
Q

Bismuth sulfate agar

A

Suppresses Caliform and allows salmonella to grow

21
Q

Dermatophyte test media

A

Terns red in the presence of dermatophytes

22
Q

What are he 3 bacterial sample collections

A

Aspiration
Swab
Imprint

23
Q

Gram stain

A

Categorizes bacteria to gram positive or gram negative

24
Q

What are the 4 steps to gram staining

A

Primary stain
Mordant
Decolorizer
Counterstain

25
Q

Gram positive color is..

A

Purple

26
Q

Gram negative color is..

A

Red

27
Q

KOH testing

A

Gram negative will develops a mucous appearance

28
Q

Ziehl neelsen stain

A

Detects acid fast organisms

29
Q

What colony characteristics do you look for?

A

Size, pigment, density, elevation, form, texture, Oder, hemolysis

30
Q

What is motility media?

A

Used if organisms are nonmotile

31
Q

What is a catalase test?

A

Performed on gram positive cocci and small gram positive bacilli

If bubbles are seen- positive

32
Q

What is the coagulase test?

A

Performed of catalase positive and gram positive cocci

If clots are seen it is positive

33
Q

What is oxidase test?

A

If you see a purple color change within a minute it is positive

34
Q

What is the California mastitis test

A

Tests for infected milk

35
Q

Mycology means

A

Fungus

36
Q

Anthropophilic

A

Lives on people

37
Q

Zoophilic

A

Lives on animals

38
Q

Geophilic

A

Lives in the environment

39
Q

What color is a positive woods lamp?

A

Apple green

40
Q

Is pasturella gram negative or gram positive?

A

Gram negative

41
Q

Is bactericides gram negative or positive?

A

Gram negative