Microbiology

Question 1

What bacteria have gram stain limitations?

Answer 1

These Rascals May Microscopically Lack Color

Treponema, Rickettsia, Mycoplasma, Mycobacteria, Legionella pneumophilia, Chlamydia

Question 2

Which bacteria stain well with Giesma stain?

Answer 2

Chlamydia, Borrelia, Rickettsiae, Trypanosomes, Plasmodium

Certain Bugs Really Try my Patience

Question 3

What are the obligate anaerobes?

Answer 3

Clostridium and Bacteriodes

Question 4

Name the obligate aerobes

Answer 4

Nocardia, Pseudomonas, Mycobacterium, Bacillus

Nagging Pests Must Breathe

Question 5

Name the obligate intracellular bugs

Answer 5

Rickettsia and Chlamydia (can’t make own ATP)

Question 6

Name the facultative intracellular bugs

Answer 6

Salmonella, Neisseria, Brucella, Mycobacterium, Listeria, Francisella, Legionella, Yersinia pestis.
(Some Nasty Bugs May Live FacultativeLY)

Question 7

What are the encapsulated bacteria?

Answer 7

Strep. pneumo, H. influenza type b, N. meningitidis, E. coli, Salmonella, Klebsiella, and Group B strep

Question 8

Catalase Positive organisms

Answer 8

Pseudomonas, Listeria, Aspergillus, Candida, E. coli, S. aureus, Serratia
(you need PLACESS for your CATs)

Question 9

Why would you conjugate a protein to a polysaccharide capsule antigen in making a vaccine?

Answer 9

To promote T-cell activation and subsequent class switching.
The polysaccharide antigen alone cannot be presented to the T cells; therefore, only IgM antibodies would be produced.

Question 10

Name the Urease-positive bugs

Answer 10

Cryptococcus, H. pylori, Proteus, Ureaplasma, Nocardia, Klebsiella, S. epidermidis, S. saprophyticus
(CHuck norris hates PUNKSS)