Microbiology

Question 1

What is the standardized media used in kirby bauer testing?

Answer 1

Mueller Hinton Agar

Question 2

What pH is MHA?

Answer 2

7.2-7.4

Question 3

You set up a KB using a MF standard of 1.0. What are the expected results of your susceptibility testing?

Answer 3

Smaller zone sizes (False resistance)

Question 4

You set up a KB using a MF standard of 0.2. What are the expected results of your susceptibility testing?

Answer 4

Larger zone sizes (false susceptibility)

Question 5

What depth should MHA be plated as?

Answer 5

3-5 mm

Question 6

What is the idea inoculum for susceptibility testing?

Answer 6

Inoculum made from 18-24 hrs old organism, pure culture made to a 0.5MF standard.

Question 7

What happens if you set up susceptibility testing on media that is 8mm deep?

Answer 7

Smaller zone sizes

Question 8

What happens if you set up susceptibility testing on media that is 2mm deep?

Answer 8

Larger zone sizes

Question 9

You want to store disks for susceptibility testing long term how should you store them?

Answer 9

-20C in a tightly sealed container with a desiccator

Question 10

You want to store disks for susceptibility testing short term how should you store them?

Answer 10

2-8C in a tightly sealed container with a desiccator