Microbiology

Question 1

Define obligate aerobe

Answer 1

An organism the only grows/divides in the presence of oxygan. Cannot survive/grow without oxygen

Question 2

Define obligate anaerobe

Answer 2

An organism that only grows/divides in the absence of oxygen. Cannot survive/divide if oxygen is present

Question 3

Define facultative anaerobe

Answer 3

An organism the can grow/divide in the presence or absence of oxygen but divides more quickly when oxygen is present

Question 4

State autoclave conditions for cultured plates and to sterilise equipment

Answer 4

121 degrees C for 15 mins

Question 5

Define total cell count

Answer 5

Counts all living and dead cells in a known volume/mass of a substance/liquid

Question 6

Define viable cell count

Answer 6

Counts all living cells in a known mass/volume of a substance/liquid.
One cell forms one colony (assumption), therefore alive. No allowance for clumping therefore underestimate