Microbiological methods Flashcards

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1
Q

why are microbiological criteria needed?

A

determine which organisms are considered hazards

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2
Q

What are standards

A
  • Document “that provides, specifications, guidelines or charcterstics that can be used consistently.
  • ISO standards – 3 years to develop
  • Inside a standard – detailed description, media composition and result expression guide
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3
Q

Detection method

A

enrichment  selective enrichment  isolation  purification  serological confirmation  biochemical confirmation

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4
Q

Advances in traditional microbiology

A
  • Chromogenic media – aids isolation of target, microorganisms and shorten analysis time
  • Petrifilms – no prep required, no use of spreaders.
  • Automation in microbiology – increase precision and accuracy
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5
Q

Rapid methods

A
  • Bioluminescence – ATP
  • Flow cytometry – characterises cells suspended in liquid based upon light absorption and scattering
  • Impedimetry – measures changes in electric properties conductivity, impedence or capacitance
  • Direct epiflurscent filter technique
  • Immunological methods – antibodies binds to a specific pathogen to enable detection in products – method ELISA
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