Microbiological methods Flashcards
1
Q
why are microbiological criteria needed?
A
determine which organisms are considered hazards
2
Q
What are standards
A
- Document “that provides, specifications, guidelines or charcterstics that can be used consistently.
- ISO standards – 3 years to develop
- Inside a standard – detailed description, media composition and result expression guide
3
Q
Detection method
A
enrichment selective enrichment isolation purification serological confirmation biochemical confirmation
4
Q
Advances in traditional microbiology
A
- Chromogenic media – aids isolation of target, microorganisms and shorten analysis time
- Petrifilms – no prep required, no use of spreaders.
- Automation in microbiology – increase precision and accuracy
5
Q
Rapid methods
A
- Bioluminescence – ATP
- Flow cytometry – characterises cells suspended in liquid based upon light absorption and scattering
- Impedimetry – measures changes in electric properties conductivity, impedence or capacitance
- Direct epiflurscent filter technique
- Immunological methods – antibodies binds to a specific pathogen to enable detection in products – method ELISA