MICROBIO-LEC -1ST LONG QUIZ (MIDTERMS) Flashcards
- English physicist
- Contributed to the discovery of cells while looking at a thin slice of cork
- This he is the first to observe cells in plant material and name them
Robert Hooke
- French Biologist
- Discovery of vaccination, microbial fermentation and pasteurization (aseptic technique)
- Produced the first vaccine for rabies by growing the virus in rabbits and then weakening it by drying the affected nerve tissue.
- Disapproved the doctrine of spontaneous generation
- Believes that “life comes from pre-existing life”
- Demonstrated that microorganisms are present in the air and can contaminate sterile solutions, but the air does not create microbes
Louis Pasteur
- Swiss physician and bacteriologist
- Discovered a bacillus which is responsible for the bubonic plague
- It was later named in his honor (Yersinia pestis)
Alexandre Emile Jean Yersin
What bacteria causes bubonic plague?
Yersinia pestis
- German physician and microbiologist
- Founder of modern microbiology
- Creation of Koch’s postulate
- Identified specific causative agents of tuberculosis,cholera and anthrax
- First person to make use of solid media
Robert Koch
What kind of solid media they used to grow the colony of bacteria way back then?
Slice of potato
This type of media adds nutrients to the bacteria
Liquid Media
- Theory of humorism
- 4 humors (black bile, yellow bile, blood and phlegm)
- Temperaments of people (bile, sanguine, phlegmatic, melancholic)
- Excess or imbalance of the different humors causes disease
Hippocrates & Galen
Complete the following:
1. Black Bile:
2. Yellow Bile:
3. Blood:
4. Phlegm:
- Melanchony (extreme sadness)
- Anger
- Cheer & courage
- Apathy
What is the full name of Paracelsus?
Philippus Aureolus Theophrastus Bombastus von Hohenheim
- Introduction of Chemistry in Medicine
- Three humors
- Salt - representing stability
- Sulphur - representing combustibility
- Mercury - representing liquidity
Paracelus
Complete the following definition:
1. Salt
2. Sulfur
3. Mercury
- Stability
- Combustibility
- Liquidity
- Essay on contagion (by contact, by fomites, at a distance)
- The name for syphilis
Girolamo Fracastoro
- Father of Microbiology
- Best known for his pioneering work in microscopy which lead the to the establishment of microbiology
- First to experiment with microbes (animalcules = “tiny animals”) and now referred as unicellular organisms
Antoine Philips Van Leeuwenhoek
- Discovered Bacillus anthracis, the causative bacterium of anthrax, in blood of diseased and dying sheep.
- Study of septicemia (blood poisoning)
Casimir Davaine
- First to observe that heating prevented the growth of bacteria in meat infusion
Lazzaro Spallanzani
- Showed that living particles can be removed from air by filtering it through cotton wool
- Introduced the use of cotton plugs in test tubes
Heinrich G. F. Schröder & Theodor von Dusch
Hypothesized that living cells arise only from preexisting living cells
Rudolf Virchow
A Scottish bacteriologist who discovered penicillin in 1928
Alexander Fleming
A Russian microbiologist who observed that viruses are filterable material; one of the founders of virology
Dmitri Ivanovski
English physician who developed vaccine against smallpox
Edward Jenner
Proposed a classification system for streptococci based on antigens in their cell walls
Rebecca Lancefield
Discovered that DNA can be transferred from one bacterium to another
Joshua Lederberg & Edward Tatum
An American biochemist and virologist who was first to characterize a virus (Tobacco Mosaic Virus)
Wendell Stanley
- A biological study that deals with the study of organisms that could not be seen by the unaided eye
- Includes the classification and general characteristics of bacteria, viruses and fungi and fundamental microbiology techniques
- Prerequisites: Botany, Zoology and Biochemistry
General Microbiology
What are the 7 disciplines in microbiology?
- Veterinary Mcrobiology
- Medical Microbiology
- Aquatc Microbiology
- Food Microbiology
- Aeromicrobiology
- Environmental Microbiology
- Molecular Microbiology
What are the 4 sub-division of microbiology and their definitions?
- Bacteriology - study of bacteria
- Virology - study of viruses
- Mycology - study of fungi (yeast and molds)
- Phycology - study of algae
The study of the role of microbes in veterinary medicine or animal taxonomy
Veterinary Microbiology
The study of the role of microbes in human illness. Includes the study of microbial pathogenesis and epidemiology and is related to the study of disease pathology and immunology
Medical Microbiology
Concerned with water purification, microbiological examination and biological degradation of wastes
Aquatic Microbiology
The study of airborne microorganisms
Aeromicrobiology
The study of the function and diversity of microbes in their natural environments
Environmental Microbiology
The study of the molecular biology and genomics of microorganisms
Molecular Microbiology
2 types of organisms
- Prokaryotic
- Eukaryotic
What are the 5 Kingdom Classification?
- Kingdom Monera (bacteria)
- Kingdom Protista (ameoba & algae)
- Kingdom Plantae (moss & ferns)
- Kingdom Animalia (insects, fish)
- Kingdom Fungi (yeast, moulds, mushrooms)
What are the differences between Prokaryotes & Eukaryotes?
Prokaryotes
- very tiny simple cells
- no true nucleus
- no organelles
Eukaryotes
- larger more complex cells
- have nucleus
- have numerous organelles
What are the differences and similarities of Autotrophs and Heterotrophs? Give at least one example.
Autotrophs
- have cell wall
- make their own food through the process of photosynthesis
- e.g. plants
Heterotrophs
- have cell wall
- can’t make their own food
- must eat other organisms
- fungi
What are the 2 types of organisms that do not have cell wall?
- Protista
- Animalia
- Disease attributed to wrath of Divine Spirits for punishment of individual sins
- Supernatural inflictions of disease
Theurgical Theory of Disease
- Due to emanations from the earth
- Influence of:
- Stars
- Moon
- Wind
- Waters
- Seasons
- Relationship of disease with waters, changes in temperature, moisture, and direction of wind
- Imbalance of (fire, air, water, earth) and four qualities (heat, cold, moisture, dryness)
- Corresponds to four humors
Miasmatic Theory of Disease
- Observation on epidemics
- Plague
- Syphilis
- Typhus in man
- contact, by fomites, at a distance
Contagion Theory (Fracastoro)
- Pasteur and Koch (lead to understanding of the presence of microorganisms and relationship to disease
- Disease is caused by infections of pathogenic microorganism
Germ Theory (18th century)
- Framework for the study of etiology or cause of infectious disease
- Pathogen must be present in every case of the disease
- The pathogen must be isolated and grown in pure culture
- The cultured pathogen must cause the disease when it is inoculated into the host of susceptible experimental host
Koch’s Postulate
It is a gradual accumulation of additional layers or matter and it is also how the rocks grow.
Accretion
What are the characteristics of livig organisms over non-living organisms
- Ability to reproduce and transmit characteristics to offspring
- Capacity to grow:
- Increase in size and volume
- Can be seen or not seen
- Internal or external growth
- Requires metabolism
- Metabolism- building process
- Catabolism- degrading process
- Non- living: growth is by accretion (gradual accumulation of additional layers or matter)
- Adaptation to environment change
- Response to stimulant (temperature, light, pressure, chemicals)
- Tropism- response towards to a stimulus
- Phototropism- response to light
- Definite body organization
- E.g. cell- tissue- organs- systems
- Definite chemical composition
- Capable of moving
- Life span and development
- Definite period of existence:
- Beginning
- Growth
- Maturity
- Decline
- Death
- Definite period of existence:
- Life cycle - Series of changes in the life of an organism including reproduction
- Constant energy requirement - For survival, development, and reproduction
Response towards to a stimulus
Tropism
Respone to light
Phototropism
use of bacteria on various industry
Biotechnology
use of either naturally occuring/ introduced microorganism or other forms of life to consume and breakdown environmental pollutants, in order to clean up polluted site
E.g. use of bacteria on toxic waste
Bioremediation
diverse collection of microorganism in the ocean or large bodies of water
Planktons
heterotrophic plankton (microscopic organism to large species) e.g. jellyfish
Zooplanktons
microscopic plants that live in the ocean
Phytoplanktons
Give the 3 types of microorganisms and their examples:
- Beneficial (87%)- e.g. antibiotics in food, chemical substances
- Opportunistic (10%)- e.g. organisms that take advantage of the weekend immune system
- Harmful (3%)- pathogens/ pathogenic organism
What are the 3 Domain System?
- Bacteria- has peptidoglycan cell wall
- Archaea- lacks peptidoglycan cell wall
- Eukarya
- Protists (slime molds, protozoa, algae)
- Fungi (unicellular yeast, multicellular molds)
- Plants (moss, ferns, flowering plants)
- Animals (worms, invertebrates, insects)