MICROBIO - Chapter 8.1 Flashcards
The aseptic collection of blood cultures requires that the skin be cleansed with:
A. 2% iodine and then 70% alcohol solution
B. 70% alcohol and then 2% iodine or an iodophor
C. 70% alcohol and then 95% alcohol
D. 95% alcohol only
B. 70% alcohol and then 2% iodine or an iodophor
When cleansing the skin with alcohol and then iodine for the collection of a blood culture, the iodine (or iodophor) should remain intact on the skin for at least:
A. 10 seconds
B. 30 seconds
C. 60 seconds
D. 5 minutes
C. 60 seconds
What is the purpose of adding 0.025% to 0.050% sodium polyanethol sulfonate (SPS) to nutrient broth media for the collection of blood cultures?
A. It inhibits phagocytosis and complement
B. It promotes formation of a blood clot
C. It enhances growth of anaerobes
D. It functions as a preservative
A. It inhibits phagocytosis and complement
A flexible calcium alginate nasopharyngeal swab is the collection device of choice for recovery of which organism from the nasopharynx?
A. Staphylococcus aureus
B. Streptococcus pneumoniae
C. Corynebacterium diphtheriae
D. Bacteroides fragilis
C. Corynebacterium diphtheriae
Semisolid transport media, such as Amies, Stuart, or Cary-Blair, are suitable for the transport of swabs for culture of most pathogens except:
A. Neisseria gonorrhoeae
B. Enterobacteriaceae
C. Campylobacter fetus
D. Streptococcus pneumoniae
A. Neisseria gonorrhoeae
Select the method of choice for recovery of anaerobic bacteria from a deep abscess.
A. Cotton fiber swab of the abscess area
B. Skin snip of the surface tissue
C. Needle aspirate after surface decontamination
D. Swab of the scalpel used for debridement
C. Needle aspirate after surface decontamination
Select the primary and differential media of choice for recovery of most fecal pathogens.
A. MacConkey, blood, birdseed, and Campylobacter (Campy) agars
B. Hektoen, MacConkey, MacConkey-Sorbitol, Campy blood, colistin–nalidixic acid (CNA) agars; Selenite-F broth (SEL)
C. CNA and Christensen urea agars and thioglycollate media
D. Blood, Campy, Mueller-Hinton agars, and thioglycollate media
B. Hektoen, MacConkey, MacConkey-Sorbitol, Campy blood, colistin–nalidixic acid (CNA) agars; Selenite-F broth (SEL)
Select the media of choice for recovery of Vibrio cholerae from a stool specimen.
A. MacConkey agar and thioglycollate media
B. Thiosulfate–citrate–bile–sucrose (TCBS) agar and alkaline peptone water (APW) broth
C. Blood agar and SEL broth
D. CNA agar
B. Thiosulfate–citrate–bile–sucrose (TCBS) agar and alkaline peptone water (APW) broth
CNA agar is used primarily for the recovery of:
A. Neisseria species
B. Enterobacteriaceae
C. Pseudomonas aeruginosa
D. Staphylococcus aureus
D. Staphylococcus aureus
In the United States, most blood agar plates are prepared with 5% or 10% red blood cells (RBCs) obtained from:
A. Sheep
B. Horses
C. Humans
D. Dogs
A. Sheep
All of the following are appropriate when attempting to isolate N. gonorrhoeae from a genital specimen except:
A. Transport the genital swab in charcoal transport medium
B. Plate the specimen directly on modified Thayer-Martin (MTM) medium
C. Plate the specimen directly on New York City or Martin-Lewis agar
D. Culture specimens in ambient oxygen at 37°C
D. Culture specimens in ambient oxygen at 37°C
Chocolate agar and MTM agar are used for the recovery of:
A. Haemophilus spp. and Neisseria spp., respectively
B. Haemophilus spp. and N. gonorrhoeae, respectively
C. Neisseria spp. and Streptococcus spp., respectively
D. Streptococcus spp. and Staphylococcus spp., respectively
B. Haemophilus spp. and N. gonorrhoeae, respectively
Cycloserine–cefoxitin–fructose agar (CCFA) is used for the recovery of:
A. Yersinia enterocolitica
B. Yersinia intermedia
C. Clostridium perfringens
D. Clostridium difficile
D. Clostridium difficile
Deoxycholate agar (DCA) is useful for the isolation of:
A. Enterobacteriaceae
B. Enterococcus spp.
C. Staphylococcus spp.
D. Neisseria spp.
A. Enterobacteriaceae
Xylose lysine deoxycholate (XLD) agar is a highly selective medium used for the recovery of which bacteria?
A. Staphylococcus spp. from normal flora
B. Yersinia spp. that do not grow on Hektoen agar
C. Enterobacteriaceae from GI specimens
D. Streptococcus spp. from stool cultures
C. Enterobacteriaceae from gastrointestinal specimens