MICROBIO Flashcards

1
Q
  1. Substrate used in Kovacs indole test:
    A. Indole
    B. Tryptophan
    C. Paradimethylaminobenzaldehyde
    D. Paradimethylaminocinnamaldehyde
A

B. Tryptophan

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2
Q
  1. Indole test differentiates which set of organisms?
    A. Morganella and Providencia
    B. Salmonella and Shigella
    C. P. vulgaris and P. mirabilis
    D. E. coli and Klebsiella oxytoca
A

C. P. vulgaris and P. mirabilis

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3
Q
  1. Inhibitors for gram-positive bacteria on MAC:
    A. Crystal violet and vancomycin
    B. Colistin and anisomycin
    C. Trimetotphrim and vancomycin
    D. Bile salts and crystal violet
A

D. Bile salts and crystal violet

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4
Q
  1. Colonies of Salmonella on SSA:
    A. Colorless colonies with black center
    B. Colorless colonies without black center
    C. Red colonies with black center
    D. Red colonies without black center
A

A. Colorless colonies with black center

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5
Q
  1. pH indicator of LIA:
    A. Neutral red
    B. Phenol red
    C. Bromothymol blue
    D. Bromocresol purple
A

D. Bromocresol purple

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5
Q
  1. Which of the following is a correct pair?
    A. S. aureus – pink colonies on MSA
    B. K. pneumoniae – colorless colonies on MAC
    C. S. pneumoniae – non hemolytic on SBA
    D. E. coli – yellow colonies on XLD
A

D. E. coli – yellow colonies on XLD

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6
Q
  1. All members of Enterobacteriaceae are motile at 37°C except:
    1) Klebsiella
    2) Proteus
    3) Shigella
    4) Yersia
    A. 1, 2, and 3
    B. 1, 3, and 4
    C. 1 and 3
    D. 1 and 4
A

B. 1, 3, and 4

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7
Q
  1. Yellow butt and red slant in TSI tube indicate fermentation of:
    A. Lactose
    B. Glucose
    C. Lactose and glucose
    D. Lactose and/or sucrose
A

B. Glucose

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8
Q
  1. Heat-stable antigen of enterics located on their cell wall:
    A. O
    B. K
    C. H
    D. Vi
A

A. O

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8
Q
  1. ONPG test allows organisms to be classified as lactose fermenter by testing which of the following enzyme:
    A. β-lactamase
    B. β-galactosidase
    C. Lactose permease
    D. Trytophanase
A

B. β-galactosidase

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9
Q
  1. Which of the following sets of results represent the most common reactions for Moraxella catarrhalis when tested in CTA sugar tubes?
    A. Glucose: Negative; Maltose: Negative; Lactose; Negative; Sucrose: Negative
    B. Glucose: Positive; Maltose: Negative; Lactose; Negative; Sucrose: Negative
    C. Glucose: Positive; Maltose: Positive; Lactose; Negative; Sucrose: Negative
    D. Glucose: Positive; Maltose: Negative; Lactose; Positive; Sucrose: Negative
A

A. Glucose: Negative; Maltose: Negative; Lactose; Negative; Sucrose: Negative

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9
Q
  1. Neisseria lactamica can be differentiated from Neisseria meningitidis through which of the following:
    A. Colony morphology
    B. Use of selective media
    C. Glucose and maltose utilization
    D. ONPG test
A

D. ONPG test

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9
Q
  1. V. cholerae colonies on TCBS:
    A. Blue
    B. Green
    C. Yellow
    D. Red
A

C. Yellow

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9
Q
  1. Only oxidase positive member of Enterobacteriaceae:
    A. Shigella
    B. Morganella
    C. Plesiomonas
    D. Edwardsiella
A

C. Plesiomonas

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10
Q
  1. Lecithinase production, double zone hemolysis on sheep blood agar, and gram-stained morphology are all useful criteria in the identification of which of the following?
    A. Clostridium perfringens
    B. Streptococcus agalactiae
    C. Escherichia coli
    D. Clostridium tetani
A

A. Clostridium perfringens

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10
Q
  1. Isolates of suspected C. diphtheriae must be tested for in vitro production of exotoxin through:
    A. Anton test
    B. Elek test
    C. Frei test
    D. Mantoux test
A

B. Elek test

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11
Q
  1. Correct about gram-positive bacteria:
    A. Has thinner peptidoglycan compared to gram-negative
    B. Cell wall has an outer layer which contains lipopolysaccharide
    C. Takes up the crystal violet-iodine complex but is easily decolorized
    D. Uses selective isolation media like Colistin Nalidixic Acid (CNA) agar
A

D. Uses selective isolation media like Colistin Nalidixic Acid (CNA) agar

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11
Q
  1. Sensitivity test result of Group A Streptococci to Bacitracin and SXT trimethoprim-sulfamethoxazole respectively:
    A. Susceptible and resistant
    B. Resistant and susceptible
    C. Both susceptible
    D. Both resistant
A

A. Susceptible and resistant

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12
Q
  1. Responsible for the dark / non-fluorescent background in auramine rhodamine stain for acid-fast bacilli
    A. Auramine
    B. Rhodamine
    C. Potassium permanganate
    D. Acid alcohol
A

C. Potassium permanganate

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13
Q
  1. 0.5 MacFarland standard provides an optical density comparable to density of a bacterial suspension of:
    A. 0.5 x 108 CFU / mL
    B. 1.5 x 108 CFU / mL
    C. 0.5 x 109 CFU / mL
    D. 1.5 x 109 CFU / mL
A

B. 1.5 x 108 CFU / mL

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13
Q
  1. Stain for endospore:
    A. Wayson
    B. Leifson
    C. Schaeffer-Fulton
    D. Loeffler’s Alkaline Methylene Blue
A

C. Schaeffer-Fulton

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13
Q
  1. In disk diffusion testing, a delay of more than 15 minutes between placing the disks on an inoculated plate and incubation results in
    A. Failure of antibiotic to diffuse in media
    B. Zones of smaller diameter will result
    C. Zones of larger diameter will result or larger zone of inhibition
    D. No effect on final zone diameter
A

C. Zones of larger diameter will result or larger zone of inhibition

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14
Q
  1. Most common gas for sterilization:
    A. Nitrogen dioxide
    B. Chlorine dioxide
    C. Ethylene oxide
    D. Chlorhexidine gluconate
A

C. Ethylene oxide

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14
Q
  1. Incorrect about autoclaving:
    A. Biologic indicator: Bacillus stearothermophilus
    B. Can destroy all forms of microbial life including spores
    C. Dry heat sterilization
    D. 121°C / 15 psi / 15 minutes
A

C. Dry heat sterilization

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15
Q
  1. Uses a strip with varying concentrations of antibiotics along its length and MIC can be obtained:
    A. Kirby Bauer
    B. Microplate dilution
    C. Vitek system
    D. E-test or elliptical test
A

D. E-test or elliptical test

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15
Q
  1. HEPA filter size used in laboratory hoods and isolation rooms
    A. 0.3 um
    B. 0.6 um
    C. 0.9 um
    D. 1.2 um
A

A. 0.3 um

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15
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15
Q
  1. The term “palisading”, “picket fence”, and “Chinese letter” describe the common arrangement of the cells of:
    A. Corynebacterium spp.
    B. Mycobacterium tuberculosis but not other mycobacteria
    C. Actinomyces but not Nocardia
    D. Corynebacterium diphtheriae but not other corynebacteria
A

A. Corynebacterium spp.

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16
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16
Q
  1. The exchange of cellular DNA between two living bacterial cells that involves an intercellular bridge is which of the following processes?
    A. Transformation
    B. Transduction
    C. Plasmidization
    D. Conjugation
A

D. Conjugation

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16
Q
  1. The slide coagulase test is a rapid screening test for the production of which of the following?
    A. Clumping factor
    B. Free coagulase
    C. Extracellular coagulase
    D. Catalase
A

A. Clumping factor

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