microbial spoilage and public health concerns part 2 Flashcards
milk is a great growth media for bacteria because of its ____, _____, and _____
high water content,
near-neutral pH,
available nutrients
why do we pasteurize milk?
to eliminate pathogens and increase shelf life
at what temperature does milk lipolysis happen faster?
4C
at what temperature does milk proteolysis happen fastest?
7C
____ are the main source of nitrogen for bacterial growth
casein proteins
what carbon is the most common carbon source in milk?
lactose, but glucose is also present in milk and serves to allow some growth of microbes
what non-protein nitrogen in milk is also readily available for microbial metabolism?
urea, peptides, and amino acids
what are the two major inhibitors of microbial growth in milk?
lactoferrin and lactoperoxidase
why is lactoferrin an inhibitor of microbial growth?
lactoferrin binds iron which makes iron a limiting factor for bacterial growth in milk
why is lactoperoxidase an inhibitor of microbial growth?
survives pasteurization. catalyzes oxidation of thiocyanate when H202 and in the process creates hypothiocyanite which is a highly reactive oxidant and is antimicrobial
what other inhibitors are there of milk?
lysozyme, specific immunoglobins, and folate and vitamin B12 binding systems
what is the limiting substrate in the lactoperoxidase system?
hydrogen peroxide
adding a hydrogen peroxide generation system to the milk increases effectiveness of the lactoperoxidase system
what two things does the preservation of milk rely on?
effective sanitation (pasteurization) and refrigeration
what is the process of pasteurizing and refrigerating milk?
raw milk is kept cold until pasteurization after which it is kept cold again until consumption
what kind of bacteria are generally the ones responsible for spoiling both raw and pasteurized milk?
psychotrophic aerobic gram-negative rods
65-70% bacteria isolated from raw milk will be ______ spp.
Pseudomonas
what other psychrotrophic bacteria are present in milk?
Aeromonas, bacillus, listeria, staphylococcus, and Enterococcus
does psychrotrophic milk spoilage rely more on proteolysis or lipolysis to spoil the milk?
proteolysis
what bacteria are most associated with milk spoilage?
pseudomonas
____ are unable to ferment lactose
pseudomonas sp.
where does pasteurized milk become contaminated?
in milk processing plant equipment and air
what causes milk spoilage by LAB?
when the milk temperature is abuse, and is stored at temperatures sufficiently high for gram-positive acid-producers to outgrow psychrotrophic
what is the effect of “wild” LAB milk spoilage?
producing unwanted gas or off flavors due to the production of acetic acid and propionic acids