microbial spoilage and public health concerns Flashcards
why is it important to understand microbial food spoilage?
to minimize economic losses and provide a high-quality food supply with adequate shelf-life
what do meat, poultry, and seafood have in common?
they are muscle foods, rich in nutrients (bacterial substrates), which allows extensive microbial growth and support the growth of bacterial pathogens
what are the differences between meat, poultry, and seafood?
They have different “original” microbiota leading to a different succession of microbial spoilage bacteria. they also differ in the handling and storage requirements, which also leads to a different microbial succession. they also differ in handling and storage requirements which also leads to a different microbial succession
under what conditions does contamination originate?
- natural: originating from the animal (GI tract, skin, feathers)
- originating from the processing environment
what is the first event in contamination?
attachment of bacterial cells to the meat surface
what bacteria is exceptionally good at attaching to meat surfaces?
pseudomonas
what is the best way to preserve meat?
having a very clean processing facility so that minimal numbers of bacteria attach to the surface of the meat
what is the significance of biofilms?
they are a major issue in food processing environments. they can form on almost any surface and can persist despite despite extensive cleaning efforts for years. once formed, bacteria can be 10- to 100- fold more resistant to sanitizers in comparison to planktonic cells
____ of all meat and __ of all fish are lost due to microbial spoilage
one fourth
30%
what conditions affect the microbial succession in meat and fish?
conditions of slaughter, decontamination, and storage
In general, _____ bacteria, mostly ______ will dominate the microflora under cold aerobic conditions, and LAB will dominate vacuum-packaged products
gram-negative
enterbacteriaceae
Pseudomonas inhibits ____and promotes ______
Shewanella
Listeria
Pseudomonas utilizes glucose and produces ____ at higher rates than Shewanella
siderophores
Pseudomonas also hydrolyses proteins and provides ____ to _____
amino acids
Listeria
what is the definition of spoilage?
consumers rejecting a food based on undesirable sensory characteristics. the definition is based on the consumer and will differ based on socio-economic factors
what does proteolytic spoilage result in?
putrid odors due to breakdown of amino acids
what does non-proteolytic spoilage result in?
sour odors
what is spoilage of canned foods due to?
improper process control, resulting in proliferation on mesophilic spore formers
what is the energy source of spoilage bacteria?
glucose
glucose is more rapidly metabolized by ____ bacteria than by ____ anaerobes
aerobic
facultative
If oxygen is present, ____ predominate in spoilage
Pseudomonas
what is the primary energy source of spoilage after glucose?
lactate, in both aerobic and anaerobic bacteria
what is the energy source after glucose and lactate?
amino acids
why are crustaceans like lobster and crayfish, kept alive until cooking?
their endogenous tissues enzymes in their hepatopancreas cause rapid postmortem muscle breakdown, independent of microbial processes
what is the best type of evaluation for quality evaluation?
sensory evaluation
the ideal spoilage indicator should…
- be absent or present in very low levels in fresh tissue
- be produced by the spoilage microflora
- increase with storage time
- correlate well with sensory analysis
What is meat traceability?
the ability to maintain credible custody of the identification of animals and their products from production to retail
the strategy for microbial control in meats is generally focused on _____ and _____
good hygiene
proper storage
methods for microbial control:
- harvest, or ship animals for slaughter with low contamination
- reduce the potential for transfer of microorganisms to carcasses, meat, and seafood from water and the environment
- apply safe and effective decontamination interventions
- apply processes such as heat, high pressure, irradiation, to reduce or eliminate microorganisms
- avoid cross-contamination
- store products at low temperature
how is the type and extent of microbial contamination determined on red meats?
determined by the hygiene of the animals before and during slaughter
common microbes on fresh red meat are ____ rods and _____
gram-negative rods
micrococci
examples of gram-negative rods and micrococci
Pseudomonas, enterobacteriaceae, acinetobacter, staphylococcus, micrococcus, coryneforms, and fecal streptococci