Microbial Metabolism [2] Flashcards

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1
Q

Due to the number of phosphate groups, ATP has approximately three times more energy stored than AMP, and ADP has approximately two-thirds the energy stored of ATP.
A True
B False

A

B

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2
Q
The Embden-Meyerhof-Parnas pathway is another name for
A the citric acid cycle.
B electron transport.
C NADH production.
D glycolysis.
A

D

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3
Q
Which of the following is a common energy storage polymer in microorganisms?
A glycogen
B adenosine triphosphate
C H2
D acetyl~S-CoA
A

A

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4
Q

From the standpoint of the microorganism, in glycolysis the crucial product is
A ethanol or lactate; ATP is a waste product.
B ATP; the fermentation products are waste products.
C CO2; ATP is a waste product.
D not relevant because glycolysis is not a major pathway.

A

B

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5
Q

Why is ATP required for glycolysis?
A ATP is used to reduce NAD+ to NADH.
B ATP is used to convert PEP into pyruvic acid.
C ATP is used to convert DHAP into G3P.
D ATP makes it easier to break apart glucose into two three-carbon molecules.

A

D

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6
Q
How many net ATPs can be made from one molecule of glucose in glycolysis?
A One
B Two
C Four
D Six
A

B

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7
Q
What carbon molecules remain at the end of glycolysis?
A Pyruvic acid
B Glyceraldehyde 3-phosphate (G3P)
C Glucose
D Dihydroxyacetone phosphate (DHAP)
A

A

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8
Q

Which of the following statements about glycolysis is true?
A All cells perform glycolysis.
B Glycolysis is also called the Embden-Meyerhof pathway.
C Glycolysis is the main source of NADH in the cell.
D Glycolysis produces glucose.

A

B

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9
Q
Glycolysis produces energy in which form?
A Pyruvic acid
B ATP
C NADH and ATP
D NADH
E NADH, ATP, and pyruvic acid
A

C

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10
Q
Which step is the step for which glycolysis is named?
A First
B Second
C Third
D Fourth
E Fifth
F Sixth
A

D

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11
Q

What is meant by substrate-level phosphorylation?
A Reduction of NAD+ to NADH
B Production of ATP using energy from a proton gradient
C Splitting of glucose into two pyruvic acid molecules
D Production of ATP by transferring phosphates directly from metabolic products to ADP

A

D

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12
Q

What is the driving force of energy production in steps 6 and 7?
A The energy potential of a proton gradient
B The reduction of three-carbon compounds
C The oxidation of three-carbon compounds
D The reduction of NAD+ to NADH

A

C

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13
Q

Electron carriers used in electron transport chains are always found in membranes. Which one of the following statements is NOT a reason why electron transport chains are found in membranes?
A So that electron-only carriers can be arranged to alternate with electron-plus-proton carriers in the chain.
B so that they can transport NADH out of the cell to the periplasm
C so that the electron carriers can be oriented within the membrane such that protons are passed from one side of the membrane to the other
D so that they can efficiently pass electrons in sequence from the carriers with the more negative reduction potentials to those with the more positive reduction potentials

A

B

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14
Q

During electron transport reactions,
A OH- accumulates on the inside of the membrane while H+ accumulates on the outside.
B both OH- and H+ accumulate on the outside of the membrane.
C OH- accumulates on the outside of the membrane while H+ accumulates on the inside.
D both OH- and H+ accumulate on the inside of the membrane.

A

A

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15
Q

How does the proton motive force lead to production of ATP?
A Translocation of three to four protons drives the F0 component of ATPase which in turn phosphorylates one ADP into ATP.
B ATPase requires one proton to make one ATP.
C Protons must be pumped against a concentration gradient from outside of the cell into the cell to rotate the F0 subunit of ATPase for the F1 subunit to make ATP.
D Oxidative phosphorylation of ADP by ATP synthase requires protons as cofactors in the reaction.

A

A

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16
Q

The proton motive force (PMF) is driven by __________.
A the difference in charge across the plasma membrane with protons outside the membrane
B the pumping of electrons though the plasma membrane
C the difference in charge across the plasma membrane with electrons outside the membrane
D the pumping of protons through the plasma membrane

A

A

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17
Q

The proton motive force is most often generated by splitting of H2.
A True
B False

A

B

18
Q

In electron transport systems, the electron carriers are membrane associated.
A True
B False

A

A

19
Q

The net result of electron transport is the generation of a pH gradient and an electrochemical potential across the membrane.
A True
B False

A

A

20
Q

Why does lack of oxygen result in the halt of ATP synthesis?
A It causes one of the cytochromes in the electron transport chain to be permanently stuck in the reduced state.
B The chain shuts down and can no longer pump hydrogen ions across the membrane, and the proton gradient cannot be maintained.
C Oxygen prevents uncoupling of the electron transport chain.

A

B

21
Q

Why might some cells uncouple the electron transport chain?
A Cells can use the energy from the proton gradient for functions other than producing ATP, such as heat generation.
B Too much ATP is bad for the cell.
C Uncoupling proteins offset the effect of cyanide.
D A cell does not require ATP.

A

A

22
Q

How does cyanide poisoning result in the decrease of ATP production?
A Cyanide uncouples the proton gradient from the process of ATP synthesis.
B Cyanide permanently reduces cytochrome a3, preventing other components to change into the oxidized state. This causes the proton gradient to break down, stopping ATP synthesis.
C Cyanide permanently binds to oxygen, preventing its use as the final electron acceptor.
D Cyanide permanently oxidizes cytochrome a3, preventing other components to change into the reduced state. This causes the proton gradient to break down, stopping ATP synthesis.

A

B

23
Q

Which of the following situations does not result in a breakdown of the proton gradient?
A Oxygen deprivation
B Uncoupling proteins
C Cyanide poisoning

A

B

24
Q
According to the animation, which compounds provide electrons to the system?
A NADH and FADH2
B FADH2
C NADH
D Water
E Oxygen
A

A

25
Q

The process of generating ATP using a proton gradient is referred to as
A the electron transport chain.
B water damming.
C chemiosmosis.

A

C

26
Q

Why does FADH2 yield less ATP than NADH?
A FADH2 electrons enter the electron transport chain at a lower energy level.
B Electrons from FADH2 cannot pump hydrogen ions out of the cell.
C FADH2 binds directly to the ATP synthase enzyme.
D FADH2 electrons ultimately do not go to oxygen.

A

A

27
Q

Which of the following can be used as a final electron acceptor for aerobic respiration?
A Molecular oxygen
B Carbonate ion
C Nitrate ion
D Nitrate ion, sulfate ion and carbonate ion can all be used as a final electron acceptor.
Sulfate ion

A

A

28
Q

What is one difference between ubiquinones and cytochromes?
A Ubiquinones can only be reduced; cytochromes can only be oxidized.
B Ubiquinones are not made of protein; cytochromes are.
C Ubiquinones cannot carry electrons; cytochromes can.
D Ubiquinones can only be oxidized; cytochromes can only be reduced.

A

B

29
Q

Iron is considered an essential element for many bacteria. Based on the animation, how would lack of iron affect energy production of a bacterium?
A Lack of iron would mean that most of the oxygen could be reduced to form water, improving energy yields.
B Lack of iron would mean lack of heme, and thus lower amounts of functioning cytochrome proteins. This would mean lower energy yields.
C Lack of iron would not have any affect on this system.

A

B

30
Q

Which intermediate compound(s) in the citric acid cycle is/are often used for biosynthetic pathways as well as carbon catabolism?
A α-ketoglutarate, oxaloacetate, and succinyl-CoA
B oxaloacetate
C α-ketoglutarate
D succinyl-CoA

A

A

31
Q

Microbial growth on the two-carbon acetate substrate invokes
A the citric acid cycle for aerobic catabolism.
B the glyoxylate and glycolysis pathways.
C the glyoxylate pathway.
D both the citric acid and glyoxylate pathways.

A

D

glyoxylate, is the TCA cycle used by some microbes, minus the steps where CO2 is lost, allows fats to become glucose

32
Q

If the citric acid cycle was interrupted after the reaction that forms citrate, predict how this would affect the total amount of ATP generated per glucose molecule.
A The amount of ATP would be reduced from a total of 38 ATP to 8 ATP.
B The amount of ATP would be reduced from a total of 38 ATP to 22 ATP.
C The amount of ATP would be reduced from a total of 38 ATP to 30 ATP.
D The amount of ATP would be reduced from a total of 38 ATP to 14 ATP.

A

D

33
Q

Regeneration of oxaloacetate is essential for the citric acid cycle to be cyclical.
A True
B False

A

A

34
Q
What occurs at the bridge step?
A Decarboxylation of pyruvic acid
B The formation of oxaloacetate
C The production of GTP
D The formation of succinyl CoA
A

A

35
Q
Based on the animation, how many electron carriers are reduced in the Krebs cycle only?
A Six
B Three
C Four
D Five
A

C

36
Q

What is the fate of metabolites during respiration?
A They are rearranged to form GTP.
B They are reduced to from NADH and FADH2.
C They are oxidized completely to carbon dioxide and water.
D They are oxidized completely to form pyruvic acid.

A

C

37
Q
Which of the following is needed as a reactant for the first step of the citric acid cycle?
A Oxaloacetic acid
B Succinyl CoA
C Malic acid
D Citric acid
A

A

38
Q
Where does the energy come from to power the formation of GTP?
A NADH
B Succinyl CoA
C Water
D ATP
A

B

39
Q
Which step involves the release of carbon dioxide?
A The third and fourth steps
B The first and eighth steps
C The second step
D The seventh step
A

A

40
Q
How many molecules of ATP can be generated from one molecule of NADH?
A One
B Four
C Three
D Two
A

C

41
Q
Which step(s) of the Krebs cycle does (do) not produce any usable energy?
A The sixth step
B The third step
C The second and seventh steps
D The first and eighth steps
E The fifth step
A

C