Microbial Genetics Flashcards

1
Q

The size of prokaryotic (bacterial) genomes is _____ (smaller/larger) than Eukaryotic genomes (human cells)

A

Smaller (about 5 million base pairs compared to about 3 billion base pairs)

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2
Q

Number of chromosomes in a prokaryotic cell?

A

One (always haploid)

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3
Q

____ typically have circular chromosomes, while ____ typically have linear chromosomes

A

Prokaryotes, eukaryotes

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4
Q

____ are small circular molecules of DNA that replicate independently. They are pretty unique to prokaryotes, but not found in all.

A

Plasmids

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5
Q

In prokaryotic cells (bacteria), chromosomes are typically found in the ____, while in eukaryotic cells they are found in _____

A

Cytosol, nucleus

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6
Q

When organisms replicate their genomes and pass them on to the next generation it is called ____ gene transfer

A

Vertical

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7
Q

When organisms exchange genes between cells of the same generation it is called ____ gene transfer

A

Horizontal

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8
Q

Name the 3 types of horizontal gene transfer used by bacteria

A
  1. Transformation
  2. Transduction
  3. Conjugation
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9
Q

What enzymes can perform DNA repair?

A

DNA Polymerases

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10
Q

A change in the nucleotide base sequence of a genome is known as:

A

Mutation

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11
Q

Name the two types of mutation and which one is most common?

A
  1. Point mutations (most common)

2. Frameshift mutations

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12
Q

Which type of mutation only affects one base pair?

A

Point mutation

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13
Q

Radiation is a mutagen. Name 1 type of ionizing radiation and 1 type of non-ionizing radiation

A

I: x-ray, gamma-ray
NI: UV rays

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14
Q

The first described representative of a species, isolated and found in nature

A

Wild type

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15
Q

Descendant of a cell that does not repair a mutation

A

Mutant

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16
Q

What test is used to test chemicals for their potential to cause mutations and potentially cancer?

A

Ames test

17
Q

What is the purpose of the Polymerase Chain Reaction (PCR)?

A

Multiplying DNA in Vitro

18
Q

Name the 3 steps of PCR

A
  1. Denaturation
  2. Priming
  3. Extension
19
Q

Intentional modification of an organism’s genomes for practical purposes

A

Recombinant DNA Technology

20
Q

Nucleic acid molecules that deliver a gene into a cell (Ex: Plasmid or Virus Bacteriophage)

A

Vectors

21
Q

Name an artificial method of recombinant DNA technology

A

Microinjection