Microbial Evolution and Systematics Flashcards
Taxonomy definition
The science of identification, classification and nomenclature
Define systematics
The study of the diversity of
organisms and their relationships
What are the three approaches of taxonomy?
Phenotypic analysis
Genotypic analysis
Phylogenetic analysis
What are some methods of genotypic analysis
DNA–DNA hybridisation
– DNA profiling
– Multilocus sequence typing
CG ratio
What is DNA-DNA hybridisation?
Genomes of two organisms are hybridized to examine proportion of similarities in their gene sequences
DNA–DNA hybridization functions
Provides rough index of similarity between two organisms
Useful for differentiating very similar organisms
Whats DNA profiling
Several methods can be used to
generate DNA fragment patterns for
analysis of genotypic similarity
among strains
Ribotyping definition
focuses on a single gene
What are GC ratios
Percentage of guanine plus cytosine
in an organism’s genomic DNA
Biological species concept definition
not meaningful as prokaryotes are haploid
and do not undergo sexual reproduction
Classification definition
Organisation of organisms into
progressively more inclusive groups
