Microbial clinical scenarios Flashcards
What is an appropriate clinical specimen to take when children involved?
- General swab of saliva or tooth surface
- minimal invasiveness
What organisms are important to detect in caries?
- Streptococcus mutans
- Lactobacillus species
What methods are available to isolate bacteria?
- Next generation sequencing
- Microbiome analysis of 16s rDNA sequences
Do you think acquiring knowledge on levels of carriage will impact overall caries for this cohort?
- Yes it has potential to
- If you establish baseline prevalence data can compare with other sites nationally/internationally
- Can be used to monitor changes over time and if significant could lead to wide range public health measures e.g. fluoridation
What preventative measures could be implemented to minimise carriage of cariogenic microorganisms?
- Improved diet advice
- behaviour advice
= Public health measures
What specimens could you investigate for patient with localised gingivits?
- Subgingival plaque biofilm
Where would you take subgingival plaque biofilm from?
- Paper point from gingival crevice on site of inflammation
What organism important in gingivitis?
- P.gingivalis
What methods can you use to identify culprit organism?
- Selective agars
- PCR
-NGS - Ideally grown on plates and undertake sensitivity testing
How do you determine cause and effect?
- Removal of culprit organism leads to resolution of disease
What is the cause of inflammation for pt with denture induced stomatitis?
- Denture associated biofilm
What organisms associated with denture induced stomatitis?
- Candida albicans
- Candida glabrata
What type of specimen could be taken to isolate organsims from denture induced stomatitis?
- Oral rinse or swab from localised area
- Foam pad for imprint
What are clinical implications of candida albicans?
- Differentiating candida albicans from candida glabrata is important as candida glabrata is insensitive to azoles
- Prescribe
Are there systemic implications of an indwelling prosthesis for pt?
- Possibility of aspiration pneumonia
- Low possible links to cardiovascular disease
What clinical specimen would you take for pt with abscess?
- Obtain pus sample
- Needle aspirate
What types of microorganism are looking for in pus sample?
- Oral anaerobes
When taking oral anaerobes specimen how should it be done?
- Obligate anaerobes are oxygen sensitive
- Need to be grown and transported in chamber with no oxygen
What techniques could be used to identify oral anaerobes?
- Standard plate culture and possibility of microscopy
Are there systemic implications if abscess not dealt with effectively?
- Spreading odontogenic infections
- Possibility of sepsis
